We narrowed to 44 results for: aen
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TypeBlog Post...Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics 198:837–846 . https://doi.org...de Bono M (2013) Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination...Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans. Genetics 210:781–787 . https://doi.org...Greatly Improves Precision Genome Editing in Caenorhabditis elegans. Genetics 216:643–650 . https://doi.org...Co-CRISPR Strategy for Efficient Genome Editing inCaenorhabditis elegans. Genetics 197:1069–1080 . https://...Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics 198:1347–1356 . https://doi.org...2013) Heritable Custom Genomic Modifications inCaenorhabditis elegansvia a CRISPR–Cas9 System. Genetics ...
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Five Popular Model Organisms
TypeBlog Post...Hesselson et al., 2009). The worm (Caenorhabditis elegans) The worm, Caenorhabditis elegans, has been used extensively...References Brenner, Sydney. "The genetics of Caenorhabditis elegans." Genetics 77.1 (1974): 71-94. PubMed...genetic interference by double-stranded RNA in Caenorhabditis elegans." nature391.6669 (1998): 806. PubMed...with cellular resolution in freely behaving Caenorhabditis elegans." Proceedings of the National Academy... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...continuously sharing their worm strains through the Caenorhabditis Genetics Center (CGC) which maintains and distributes...high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics 202.4 (2016): 1277-1288....cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017): 145. ...refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings of the National Academy... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...scattering. The best candidate for this was the Anabaena flos-aquae gas vesicle gene cluster because of... increase expression. We were able to express Anabaena GVs in mammalian cells without the shell stiffening...nonlinear ultrasound contrast. It turned out that Anabaena mARGs did not require a booster cassette, either... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...modifier, Fuw-dCas9-Tet1CD, is available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus...Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf Jaenisch’s lab, with the former also available as ready-..., Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing DNA Methylation in the Mammalian... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post... Rebar, E. J., Gregory, P. D., Zhang, H. S., & Jaenisch, R. (2011). Generation of isogenic pluripotent...S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R. (2013). One-Step generation of mice carrying...H., Shivalila, C. S., Cheng, A. W., Shi, L., & Jaenisch, R. (2013). One-Step generation of mice carrying... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous...linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Paix A, Wang Y, Smith HE, Lee CY, ... -
Hot Plasmids - October 2020
TypeBlog Post...Split-wrmScarlet, a fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins...proteins (FPs) traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to ... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step Generation of Mice Carrying ..., Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R (2013) One-Step Generation of Mice Carrying ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis elegans. They have found several voltage biosensors... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...Hans Schöler, and closing keynote speaker Rudolph Jaenisch have also spoken at this meeting. In fact, we ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391 (1998), pp. 806-811. PubMed... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...fluorescent labeling of endogenous proteins in Caenorhabditis elegans. Goudeau et al. Genetics. 2021 Cynthia... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...to study many different mutants because the Caenorhabditis Genetics Center (CGC) at the University of ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...26726264. PubMed Central PMCID: PMC4686367. 2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88. ... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab. Figure 3: A knockout/knock-in targeting... -
To Codon Optimize or Not: That is the Question
TypeBlog Post... EM, Lester HA (2003) Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...spp., Alcaligenes spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp... -
Hot Plasmids: Fall 2024
TypeBlog Post... new mScarlet-based red fluorescent tags in Caenorhabditis elegans. Genetics, 228(2), iyae126. https:/...