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Showing: 1 - 20 of 26 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics 198:837–846 . https://doi.org...de Bono M (2013) Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination...Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans. Genetics 210:781–787 . https://doi.org...Greatly Improves Precision Genome Editing in Caenorhabditis elegans. Genetics 216:643–650 . https://doi.org...Co-CRISPR Strategy for Efficient Genome Editing inCaenorhabditis elegans. Genetics 197:1069–1080 . https://...Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics 198:1347–1356 . https://doi.org...2013) Heritable Custom Genomic Modifications inCaenorhabditis elegansvia a CRISPR–Cas9 System. Genetics ...

  2. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...Hesselson et al., 2009). The worm (Caenorhabditis elegans) The worm, Caenorhabditis elegans, has been used extensively...References Brenner, Sydney. "The genetics of Caenorhabditis elegans." Genetics 77.1 (1974): 71-94. PubMed...genetic interference by double-stranded RNA in Caenorhabditis elegans." nature391.6669 (1998): 806. PubMed...with cellular resolution in freely behaving Caenorhabditis elegans." Proceedings of the National Academy...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...continuously sharing their worm strains through the Caenorhabditis Genetics Center (CGC) which maintains and distributes...high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics 202.4 (2016): 1277-1288....cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017): 145. ...refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings of the National Academy...

  4. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...scattering. The best candidate for this was the Anabaena flos-aquae gas vesicle gene cluster because of... increase expression. We were able to express Anabaena GVs in mammalian cells without the shell stiffening...nonlinear ultrasound contrast. It turned out that Anabaena mARGs did not require a booster cassette, either...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...modifier, Fuw-dCas9-Tet1CD, is available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus...Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf Jaenisch’s lab, with the former also available as ready-..., Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing DNA Methylation in the Mammalian...

  6. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous...linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Paix A, Wang Y, Smith HE, Lee CY, ...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Split-wrmScarlet, a fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins...proteins (FPs) traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to ...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-Step Generation of Mice Carrying ..., Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R (2013) One-Step Generation of Mice Carrying ...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout/knock-in targeting...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... new mScarlet-based red fluorescent tags in Caenorhabditis elegans. Genetics, 228(2), iyae126. https:/...

  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...spp., Alcaligenes spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp...
Showing: 1 - 20 of 26 results