-
Plasmid#180319Purposemammalian expression of human SEPT2 fused to monomeric AppleDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationfull-lengthPromotertac promoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
OPEN HLA-A*02:01-BirA
Plasmid#215569PurposeE. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.DepositorInsertOPEN HLA-A*02:01 (HLA-A Human)
UseTagsBirAExpressionBacterialMutationGlycine 120 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
XIAP (pBS0852)
Plasmid#185282PurposeFor the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.DepositorInsertXIAP (XIAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
psigma2-EGFP
Plasmid#53610PurposeExpresses sigma2 of adaptor protein of AP2DepositorInsertsigma2 (Ap2s1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCcnb2
Plasmid#162794PurposeCcnb2 shRNA in pMKO.1 retroviral vectorDepositorInsertCcnb2 shRNA (Ccnb2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shControl
Plasmid#180386PurposeProducing AAV that encodes negative control shRNA with miR-E backboneDepositorInsertshControl
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
UseTagsExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKT2/CLP-AKT
Plasmid#20281DepositorInsertAKT (AKT1 Human)
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hAtg13
Plasmid#22872DepositorInsertautophagy-related genes 13 (ATG13 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_AchLightG
Plasmid#187464PurposeExpresses green acetylcholine indicator AchLightG in neuronal cellsDepositorInsertAchLightG
UseAAVTagsIgκ leaderExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 p18 shRNA
Plasmid#26631DepositorInsertp18 shRNA (LAMTOR1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_G3BP1_G1
Plasmid#127114DepositorInsertgRNA G3BP1 (G3BP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-nCas9
Plasmid#163548PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-MYC
Plasmid#130695PurposeLentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.DepositorInserthuman MYC (MYC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-nosT
Plasmid#71272PurposeEntry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertnopaline synthase terminator
UseGatewayTagsExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeTagsExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only