We narrowed to 12,952 results for: sequence
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Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-a-element-L4
Plasmid#186366PurposeEntry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequencesDepositorInserta-element early Otx neural enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-P(11)4
Plasmid#185786PurposeEncodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ATA-P(11)4
Plasmid#185787PurposeEncodes enzyme ω-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertATA-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-P(11)4
Plasmid#185789PurposeEncodes glutathione S-transferase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertGST-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-myc-GRM3 C127A-VC
Plasmid#140450PurposeMammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alaninePromoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato--β-actin CDS2
Plasmid#183714PurposetdTomato with fragment nucleotides 398-818 of beta-actin coding sequence inserted in 3'UTRDepositorInserttdTomato with fragment (nucleotides 398-818) of mouse Actb coding dsequence
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6A_L
Plasmid#146843PurposeInsect Expression of HsTNRC6ADepositorInsertHsTNRC6A (TNRC6A Human)
UseTagsExpressionInsectMutationA339T, K1578E compared to the sequence of isoform…PromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin
Plasmid#182305PurposeE. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.DepositorInsertpH-Countdown insertion into GECO 1.2 calcium sensing domains
UseTagsA modified TorA periplasmic targeting sequence pl…ExpressionBacterialMutationSpontaneous mutation of calmodulin's penulti…Promotert7Available SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F
Plasmid#146286PurposeInsect Expression of HsAGO2-F2V2DepositorInsertHsAGO2-F2V2 (AGO2 Human)
UseTagsExpressionInsectMutationContains F470V, F505V, K660E mutations compared t…PromoterAvailable SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V
Plasmid#147767PurposeMammalian Expression of HsSmg5-G120E-del133-155DepositorInsertHsSmg5-G120E-del133-155 (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S
Plasmid#147470PurposeMammalian Expression of HsSmg5-G120E-siRNAresDepositorInsertHsSmg5-G120E-siRNAres (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R
Plasmid#147407PurposeInsect Expression of CeAIN2_254-400DepositorInsertCeAIN2_254-400
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_A
Plasmid#145893PurposeInsect Expression of DmHPatDepositorInsertDmHPat (Patr-1 Fly)
UseTagsExpressionInsectMutationthree silent mutations compared to the sequence g…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)
Plasmid#170488PurposeSPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1DepositorInsertFRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-MTS-GFP11-Ura
Plasmid#177733PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA markerDepositorInsertMTS-GFP11
UseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-Ck1-hs1-l-chlo
Plasmid#163937PurposeFor integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha trnG_trnfM region left homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck1-hs2-l-chlo
Plasmid#163938PurposeFor integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha rbcL_trnR region homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck4-hs1-r-chlo
Plasmid#163940PurposeFor integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha trnG_trnfM region right homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck4-hs2-r-chlo
Plasmid#163941PurposeFor integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha rbcL_trnR region right homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEPQD0CM0705
Plasmid#177035PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertphiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Ovol1
Plasmid#172871PurposeMammalian expression vector with myc-tagged mouse Ovol1 coding sequenceDepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits