We narrowed to 62,158 results for: cloning
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Plasmid#86409PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSPromoterAtRPS5AAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise-MLC-IRES-neo
Plasmid#85145Purposelentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoiseDepositorInsertmTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
UseLentiviralTagsmTurquoisePromoterEF1aAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD2
Plasmid#85658Purposeretroviral expression of human SMYD2DepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti Dest BlastR R1-R2
Plasmid#84574PurposeGateway destination vector expressing Blasticidin resistance under a PGK promoterDepositorTypeEmpty backboneUseGateway Cloning and LentiviralExpressionMammalianPromoterPGKAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416-Gal-PR50
Plasmid#84901Purposeinduce expression of dipeptide repeat PR in yeastDepositorAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAP1 C91A
Plasmid#81025PurposeRetroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection markerDepositorInsertBAP1 (BAP1 Human)
UseRetroviralExpressionMammalianMutationBAP1 C91A (point mutation, catalytically inactive)Promoter5’LTRAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9
Plasmid#84243PurposeExpresses ABA-inducible VPR-Sa dCas9DepositorInsertsABI-tagBFP-Sa dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionPromoter-3mnx1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-hMYOD1-3xHA (#730)
Plasmid#78328PurposeDoxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tagDepositorAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB102
Plasmid#68556PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YN
Plasmid#74721PurposeExpress C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantPromoterCaMV 35SAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRExpressionInsectPromoterdU6:3Available SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ151
Plasmid#69302PurposeGateway entry vector with pcoCas9D10ADepositorInsertpcoCas9D10A (Plant codon-optimized)
UseCRISPRTags2XFLAGMutationD10A mutation; NLS fusionAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestattB/CY (pCM327)
Plasmid#68341PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.DepositorTypeEmpty backboneUseZebrafish transgenesisAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestattB_ubi:EGFP (pCM272)
Plasmid#68339PurposeMultisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.DepositorInsertEGFP
UseZebrafish transgenesisPromoterubiAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC13P-iCLHN
Plasmid#66580PurposeCLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENsDepositorInsertNanoluc-HaloTag
ExpressionMammalianPromoterCAGAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Kras (G12V)-pcw107
Plasmid#64602Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only