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pJC-repo1.9kb-TALE4-VP64-P10

Plasmid

#104612

Purpose

Expresses TALE4 under the control of a 1.9kb repo enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.9kb repo enhancer element (repo Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PHC3

Plasmid

#45262

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PHC3 (PHC3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

IRFP and LEXY

Expression

Insect

Promoter

maternal tubulin

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAFW-Ago2

Plasmid

#50554

Purpose

Expression of FLAG tagged Ago2 in S2 cell

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

Insert

Argonaute2 (AGO2 Fly)

Tags

FLAG

Expression

Insect

Promoter

Actin5C

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO PYL1 (36-211)

Plasmid

#177843

Purpose

Bacterial Expression of PYL1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 1 (PYL1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111547

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-3xHA-V5-RpL10Ab

Plasmid

#125223

Purpose

3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

3x HA, V5

Expression

Insect

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-Brn2-DBD-EnR

Plasmid

#19714

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Fly engrailed 36240 (Pou3f2, en Fly, Mouse)

Expression

Mammalian

Mutation

mouse Brn2 DNA binding domain-Drosophila engraile…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pAc-GFP-dSUMO

Plasmid

#85696

Purpose

expression of GFP-SUMO1 in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Tags

GFP

Expression

Insect

Promoter

Ac5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only