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A multiplexable TALE-based binary expression system for in vivo cellular interaction studies.
Toegel M, Azzam G, Lee EY, Knapp DJHF, Tan Y, Fa M, Fulga TA
Nat Commun. 2017 Nov 21;8(1):1663. doi: 10.1038/s41467-017-01592-3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104605pJC-20xUAS-TALE-VP64-P10Control plasmid (no TALE array) with P10 terminator
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104606pJC-20xUAS-TALE1-VP64-P10Expresses TALE1 under UAS control
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104607pJC-20xUAS-TALE3-VP64-P10Expresses TALE3 under UAS control
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104608pJC-20xUAS-TALE4-VP64-P10Expresses TALE4 under UAS control
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104609pJC-elav1.8kb-TALE1-VP64-P10Expresses TALE1 under the control of a 1.8kb elav enhancer element
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104610pJC-ase0.8kb-TALE3-VP64-P10Expresses TALE3 under the control of a 0.8kb ase enhancer element
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104611pJC-mhc2.4kb-TALE3-VP64-P10Expresses TALE3 under the control of a 2.4kb mhc enhancer element
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104612pJC-repo1.9kb-TALE4-VP64-P10Expresses TALE4 under the control of a 1.9kb repo enhancer element
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104613pJFRC81-3xVAS1-IVS-Syn21-GFP-P10Expresses GFP upon binding of TALE1 to VAS1
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104614pJFRC81-3xVAS3-IVS-Syn21-GFP-P10Expresses GFP upon binding of TALE3 to VAS3
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104615pJFRC81-3xVAS4-IVS-Syn21-GFP-P10Expresses GFP upon binding of TALE4 to VAS4
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104616pJFRC81-3xVAS1-Syn21-Citrine-HA-P10Expresses HA tagged Citrine upon binding of TALE1 to VAS1
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104617pJFRC81-3xVAS3-Syn21-V5-mCherry-P10Expresses V5 tagged mCherry upon binding of TALE3 to VAS3
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104618pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4
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104619pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4
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Recombinant Antibodies from Article