We narrowed to 18,395 results for: Tec

1. pGWB401

   Plasmid

   #74795

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShHELIX_sgRNA_entry (CJT32)

   Plasmid

   #181782

   Purpose

   Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTMV

   Plasmid

   #118755

   Purpose

   Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB451

   Plasmid

   #74833

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK419 (GAL-OsTIR1(F74G))

   Plasmid

   #140656

   Purpose

   GAL-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB411

   Plasmid

   #74805

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR0306 CcaCas13b His6-TwinStrep-SUMO

   Plasmid

   #182687

   Purpose

   Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   CcaCas13b
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MMLV-RT(dRH) for Split-PE (pEH50)

   Plasmid

   #190106

   Purpose

   Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT(delta RNAseH)
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGM1202

    Plasmid

    #69615

    Purpose

    inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-tetO-NFIA

    Plasmid

    #141403

    Purpose

    Inducible Expression of NFIA

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

    Insert

    NFIA (NFIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWB517

    Plasmid

    #74859

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.4_17G6-1 heavy chain

    Plasmid

    #198249

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ND4.2-Left TALE-G1397-N-dead-mCherry

    Plasmid

    #179683

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1347A

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.4_17G6-1 light chain

    Plasmid

    #198250

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19/ER-mChe

    Plasmid

    #183163

    Purpose

    Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    ER-mCherry
    

    Use

    Tags

    25 aa ER signal of Glycine soja kunitz-type tryps…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB514

    Plasmid

    #74856

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB551

    Plasmid

    #74881

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PHP.AX

    Plasmid

    #195218

    Purpose

    AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

    Depositor

    Viviana Gradinaru

    Article

    Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

    Insert

    Synthetic construct isolate AAV-PHP.AX VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 35S-GWY-VP16/pAM

    Plasmid

    #201232

    Purpose

    Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GWY-VP16
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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