We narrowed to 165,385 results for: addgene

1. pCY5.1nh

   Plasmid

   #160299

   Purpose

   Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCY5.1kn

   Plasmid

   #160298

   Purpose

   Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. NOLC1 atgRNA pDY2250

   Plasmid

   #219860

   Purpose

   attachment site pegRNA for human NOLC1

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. NOLC1 nicking guide pDY2251

   Plasmid

   #219861

   Purpose

   Nicking sgRNA for NOLC1

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 nicking sgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEIF3D-WT-sgNT

   Plasmid

   #222120

   Purpose

   EIF3D overexpression vector with non-targeting sgRNA

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   eIF3D (EIF3D Human)
   

   Use

   Lentiviral

   Tags

   BFP-P2A

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHW1387

   Plasmid

   #229880

   Purpose

   Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
   

   Tags

   GFP-C1

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHW1392

   Plasmid

   #229878

   Purpose

   Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Insert

   Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
   

   Tags

   GFP-C1

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-CALU-WPRE-UbC-Emerald

   Plasmid

   #225951

   Purpose

   Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CALU (CALU Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-CALU-WPRE-UbC-mCherry

   Plasmid

   #225952

   Purpose

   Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CALU (CALU Human)
   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-LNPK-WPRE-UbC-Emerald

    Plasmid

    #225953

    Purpose

    Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Evan Reid, Bart Nieuwenhuis

    Inserts

    LNPK (LNPK Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-LNPK-WPRE-UbC-mCherry

    Plasmid

    #225954

    Purpose

    Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Evan Reid, Bart Nieuwenhuis

    Inserts

    LNPK (LNPK Human)
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGK58

    Plasmid

    #228118

    Purpose

    Express node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKSEe401R

    Plasmid

    #218532

    Purpose

    CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.

    Depositor

    Kirill Demchenko

    Article

    Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tensin-2 Y483E

    Plasmid

    #201794

    Purpose

    Expresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483E

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tensin-2 Y483F

    Plasmid

    #201795

    Purpose

    Expresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483F

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tensin-2 Y483A

    Plasmid

    #201796

    Purpose

    Expresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483A

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGK65

    Plasmid

    #228123

    Purpose

    Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGK59

    Plasmid

    #228119

    Purpose

    Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGK57

    Plasmid

    #228117

    Purpose

    Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge