We narrowed to 7,529 results for: Ski;

1. nSaCas9 H119 miniABEMax V82G

   Plasmid

   #135381

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9-ABEMax(V82G) H119
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. nSaCas9 M732 miniABEMax V82G

   Plasmid

   #135382

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 M732 miniABEMax V82G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nSaCas9 M741 miniABEMax V82G

   Plasmid

   #135383

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 M741 miniABEMax V82G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nSaCas9 N120 miniABEMax V82G

   Plasmid

   #135384

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 N120 miniABEMax V82G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. S2 1058 AIDx no UGI

   Plasmid

   #135349

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. S2 1058 APOBEC3 no UGI

   Plasmid

   #135350

   Purpose

   Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-rAPOBEC3-ID-1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. S1 1029 AIDx with UGI

   Plasmid

   #135354

   Purpose

   Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   hAIDx-nSpyCas9 aa-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. S2 1058 ABEMax

   Plasmid

   #135356

   Purpose

   Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-ABEMax aa 1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. S1 1029 CP ABEMax strategy 3

   Plasmid

   #135359

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 CP nSpyCas9 ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. S1 1029 miniABEMax V82G strategy1

    Plasmid

    #135360

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    mini ABEMax 1029 nSpyCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. S2 1058 V82G ABEMax

    Plasmid

    #135361

    Purpose

    Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    1058 nSpyCas9 V82G ABEMax
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. S1 1029 V82G ABEMax Strategy 3

    Plasmid

    #135363

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    1029 V82G ABEMax
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AIDx-ID-202

    Plasmid

    #135344

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-202
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-FRT-TO-Nprot_GC3opt

    Plasmid

    #157730

    Purpose

    mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot_GC3opt (N SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA5-FRT-TO-Nsp8_GC3opt

    Plasmid

    #157697

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

    Plasmid

    #157700

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA5-FRT-TO-FH-Nsp8_GC3opt

    Plasmid

    #157698

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA3.1/NDUFS2 _2-33/myc-His

    Plasmid

    #127492

    Purpose

    Expresses myc-tagged _MTS NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _2-33 (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _2-33

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/NDUFS2 _2-78/myc-His

    Plasmid

    #127493

    Purpose

    Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _2-78 (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _2-78

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRAV29

    Plasmid

    #123401

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV29 (TRAV29DV5 Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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