We narrowed to 14,036 results for: TIM

1. PTK2 gRNA (BRDN0001145734)

   Plasmid

   #75543

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PTK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PTK2 (PTK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIB2 gRNA (BRDN0001144754)

   Plasmid

   #75602

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB2 (TRIB2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIB2 gRNA (BRDN0001148141)

   Plasmid

   #75603

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB2 (TRIB2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-GRAB_ACh3.0

   Plasmid

   #121923

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSuper-Retro-Puro-Drp1-shRNA

   Plasmid

   #99385

   Purpose

   Expresses shRNA against human Drp1 from puromycin resistance retroviral vector

   Depositor

   David Kashatus

   Article

   Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

   Insert

   Dynamin-1-like protein (DNM1L Human)
   

   Use

   RNAi and Retroviral

   Promoter

   H1

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-Spy-CD86

   Plasmid

   #223598

   Purpose

   Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Spytag003

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-OGT_opt

   Plasmid

   #190858

   Purpose

   For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells.

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pHR-SNAP-PDL1

   Plasmid

   #223602

   Purpose

   Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJR100

   Plasmid

   #187240

   Purpose

   Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
   

   Use

   Lentiviral

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRES proHB-EGF NC

    Plasmid

    #11604

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

11. fluoPEER-HEK3_5bp-del

    Plasmid

    #185476

    Purpose

    Fluorescent reporter for a 5 basepair deletion at the HEK3 locus.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3 editing site
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2K7-bsd-UBI-tagRFP-KDEL

    Plasmid

    #114179

    Purpose

    Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Lentiviral

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVAX1-cGAS

    Plasmid

    #196624

    Purpose

    Express mouse cGAS protein in mammalian cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    cGAS (Cgas Mouse)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO-Hand2

    Plasmid

    #46028

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    Hand2 (Hand2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. FKBP:FRB-FBAR

    Plasmid

    #223620

    Purpose

    Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    FBP17 (FNBP1 Human)
    

    Tags

    FKBP, TagBFP and mGFP, FRB

    Expression

    Mammalian

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-HA-FbxO5-pcDNA3.1-

    Plasmid

    #52509

    Purpose

    expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO5 (FBXO5 FbxO5, Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166012

    Purpose

    mammalian expression of SARS-CoV soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV S (spike)
    

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-mGFP-Eps15DN

    Plasmid

    #223621

    Purpose

    Transient expression of human Eps15 with mGFP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    Eps15 (EPS15 Human)
    

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Eps15 _ 95-295aa

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRES proHB-EGF CS

    Plasmid

    #11603

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Expression

    Mammalian

    Mutation

    Constitutively secreted HB-EGF: C-terminal amino …

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only