Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,921 results for: pET

Clear keywords
Showing: 5321 - 5340 of 9921 results

  3. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162681-plasmid-map-sequence-id-321444

    Enlarge

  4. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162682-plasmid-map-sequence-id-320836

    Enlarge

  6. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

    Enlarge

  7. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

    Enlarge

  8. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

    Enlarge

  10. FGFR1_HUMAN_D0

    Plasmid
    #79719
    Purpose
    This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    FGFR1 (FGFR1 Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  12. CDK2_HUMAN_D0

    Plasmid
    #79726
    Purpose
    This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    CDK2 (CDK2 Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  13. VRK1_HUMAN_D0

    Plasmid
    #79684
    Purpose
    This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    VRK1 (VRK1 Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    79684-plasmid-map-sequence-id-513103

    Enlarge

  16. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Publication
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

    Enlarge

  19. WT-eGFP

    Plasmid
    #215168
    Purpose
    Encodes the enhanced green fluorescent protein
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    WT-eGFP
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215168-plasmid-map-sequence-id-430164

    Enlarge

  20. His-GST

    Plasmid
    #215169
    Purpose
    Encodes the synthetic glutathionine S-transferase with N-terminal His tag
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    His-GST
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215169-plasmid-map-sequence-id-430165

    Enlarge

Showing: 5321 - 5340 of 9921 results