We narrowed to 15,480 results for: pET

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b

Plasmid

#182276

Purpose

Expresses recombinant BrCas12b

Depositor

Piyush Jain

Article

Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis, T7, Thrombin site

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pNS20-SpCas9-SNAP

Plasmid

#113717

Purpose

Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G175R)-Strep

Plasmid

#196211

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G175R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G189E)-Strep

Plasmid

#196212

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G189E

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNS38-SadCas9-SNAP

Plasmid

#113718

Purpose

Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

D10A, N580A

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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His10-CD3z-SNAPf-ybbR (XSB507)

Plasmid

#78508

Purpose

Construct for expressing CD3z-SNAPf-ybbR in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD3z (Cytoplasmic domain) (CD247 Human)

Tags

His10 and SNAPf-ybbR

Expression

Bacterial

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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FAK2_HUMAN_D0

Plasmid

#79747

Purpose

This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FAK2 (PTK2B Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK24_HUMAN_D0

Plasmid

#79742

Purpose

This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK24 (STK24 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
ZIKV_Trigger_27B

Plasmid

#75008

Purpose

Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_27B

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2017

Availability

Academic Institutions and Nonprofits only

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MEF2A-Sensor

Plasmid

#208928

Purpose

in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

Mef2a docking sequence+Phospho site (MEF2A Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_32B

Plasmid

#75009

Purpose

Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_32B

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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SLK_HUMAN_D0

Plasmid

#79733

Purpose

This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SLK (SLK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
ZIKV_NASBA_32B

Plasmid

#75011

Purpose

Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_32B

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_27B

Plasmid

#75010

Purpose

Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_27B

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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STK16_HUMAN_D0

Plasmid

#79701

Purpose

This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK16 (STK16 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CSK_HUMAN_D0

Plasmid

#79683

Purpose

This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK (CSK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pJTB115

Plasmid

#248909

Purpose

Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria

Depositor

Edward Winter

Article

Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-

Inserts

SMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)

Tags

glutathione-S-transferase

Expression

Bacterial

Mutation

NdeI and NcoI sites were removed for cloning purp…

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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L4428 IL-10Ra CTEVp chain with Tbet in TUPV3

Plasmid

#244196

Purpose

Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hTbet

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and human Tbet (TBX21 Synthetic, Human)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

CTEVp_190K

Promoter

hEF1a

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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