We narrowed to 6,558 results for: sas;

1. pcDNA1-Tat

   Plasmid

   #138478

   Purpose

   Expresses HIV Tat for efficient sgRNA packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Tat
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xMx PylT_FLAG-Mx PylRS

   Plasmid

   #140011

   Purpose

   Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HyperADAR control

   Plasmid

   #166969

   Purpose

   Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

   Depositor

   Michael Kharas

   Article

   Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

   Insert

   linker-hyperADAR
   

   Use

   Retroviral

   Tags

   linker-HyperADAR

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-HAT1_AG

   Plasmid

   #217113

   Purpose

   Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   HAT1_AG transposase
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-CAG-hCas3

   Plasmid

   #134920

   Purpose

   Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas3 with bpNLS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Mariner2_AG

   Plasmid

   #217135

   Purpose

   Mammalian expression vector for expression of Mariner2_AG transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   Mariner2_AG transposase
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV.5’-LMNA-eGFP-KI

   Plasmid

   #170545

   Purpose

   Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Inserts

   Left Homology arm for LMNA knockin
   Right Homology arm for VIM knockin
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. KillerRed-LacI-NLS

   Plasmid

   #215102

   Purpose

   Expresses KillerRed-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   KillerRed-LacI-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR62-Tango

   Plasmid

   #66367

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR62 (GPR62 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pPing

    Plasmid

    #47100

    Purpose

    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    Ping cDNA
    mPing
    hygromycin resistance gene
    

    Use

    Plant expression

    Tags

    GFP

    Expression

    Yeast

    Promoter

    CaMV35s and StUbi3

    Available Since

    Sept. 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. GPR4-Tango

    Plasmid

    #66358

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR4 (GPR4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCold-TF-hRubicon

    Plasmid

    #64147

    Purpose

    For protein expression in E. coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    Rubicon (RUBCN Human)
    

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pGEX6P1 p37

    Plasmid

    #169015

    Purpose

    Expresses GST-p37 (human, codon optimized for expression in bacteria)

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    p37 (UBXN2B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    sequence was codon optimized for expression in E.…

    Promoter

    tac

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR141-Tango

    Plasmid

    #66318

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR141 (GPR141 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pUPD2 RDF ( GB1498)

    Plasmid

    #160579

    Purpose

    Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Promoter

    EF1a

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. P2RY2-Tango

    Plasmid

    #66474

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    P2RY2 (P2RY2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. peGFP-HDAC1 (pc2447)

    Plasmid

    #240148

    Purpose

    Expression vector containing a fusion protein of eGFP and human HDAC1.

    Depositor

    Cristina Cardoso

    Article

    Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

    Insert

    HDAC1 (HDAC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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