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pD(P+T)_T7C

Plasmid

#223856

Purpose

4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7

Plasmid

#223854

Purpose

4G-cloning donor for bacterial promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

T7 promoter and terminator

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His10-TS-3C

Plasmid

#223884

Purpose

4G-cloning donor for N-terminal His10-TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His10-TwinStrep-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-SUMO

Plasmid

#223879

Purpose

4G-cloning donor for N-terminal His6-SUMO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-SUMO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-TEV

Plasmid

#223881

Purpose

4G-cloning donor for N-terminal GFP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-GFP

Plasmid

#223888

Purpose

4G-cloning donor for C-terminal 3C-GFP tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-GFP

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-His8

Plasmid

#223887

Purpose

4G-cloning donor for C-terminal 3C-His8 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_CPD-His10

Plasmid

#223890

Purpose

4G-cloning donor for C-terminal CPD-His10 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

CPD-His10

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'alpha

Plasmid

#223863

Purpose

4G-cloning donor for 5'-Gibson overhang, alpha-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'alpha sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'gamma

Plasmid

#223865

Purpose

4G-cloning donor for 5'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'gamma sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'beta

Plasmid

#223864

Purpose

4G-cloning donor for 5'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'beta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'delta

Plasmid

#223866

Purpose

4G-cloning donor for 5'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'delta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'beta

Plasmid

#223867

Purpose

4G-cloning donor for 3'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'beta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'gamma

Plasmid

#223868

Purpose

4G-cloning donor for 3'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'gamma sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'delta

Plasmid

#223869

Purpose

4G-cloning donor for 3'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'delta sequence and spacer

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSpy

Plasmid

#220184

Purpose

sgRNA expression vector - promoterless backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

promoterless backbone

Use

CRISPR

Expression

Bacterial

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA

Plasmid

#220185

Purpose

sgRNA expression vector - pEM7 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pEM7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD

Plasmid

#220188

Purpose

sgRNA expression vector - pBG28 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG28

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_NAP1-eGFP

Plasmid

#216837

Purpose

For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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