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Membrane protein gRNA library

Pooled Library

#113345

Purpose

Activation library targeting the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform.

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Species

Homo sapiens

Use

Lentiviral

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

Pooled Library

#83976

Purpose

Human CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
TCR Rapid Assembly for Functional Testing (TCRAFT)

Pooled Library

#1000000264

Depositor

Michael Birnbaum

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

Pooled Library

#83994

Purpose

Mouse CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

Pooled Library

#83985

Purpose

Human CRISPRa Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

Pooled Library

#84003

Purpose

Mouse CRISPRa Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins

Pooled Library

#101929

Purpose

Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.

Depositor

Michael Bassik

Article

Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

Expression

Mammalian

Use

CRISPR

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Membrane proteins

Pooled Library

#1000000124

Purpose

CRISPR gRNA mouse knockout pooled library - Membrane proteins

Depositor

Michael Bassik

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

Plasmid

#85434

Purpose

Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab

Use

Lentiviral

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only
ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCLASP_mScarlet_CLASP_RGS2membrane

Plasmid

#133086

Purpose

Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

mScarlet-CLASP

Expression

Bacterial and Yeast

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Assembled_PULTRA_GFP_RHOA_WT

Plasmid

#252256

Purpose

Express GFP-RHOA

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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Assembled_PULTRA_GFP_RHOA_Y42C

Plasmid

#252257

Purpose

Express GFP-RHOA(Y42C)

Depositor

David Chan

Article

Yang et al Nat Commun. 2025 Dec 14;16(1):11260. doi

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

RHOA Y42C

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pRembiv

Plasmid

#252216

Purpose

Yeast expression of BK polyomavirus VP1 and aeBlue reporter

Depositor

Christopher Buck

Inserts

BKV-IV VP1
aeBlue
Formaldehyde dehydrogenase 1

Expression

Yeast

Promoter

FDH1 and MAL32

Available Since

Feb. 23, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-pmEMBer

Plasmid

#174441

Purpose

Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEMBer

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-Embigin

Plasmid

#221417

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

Embigin (EMB Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1-TandemBRCT

Plasmid

#250354

Purpose

Expresses tandem BRCT domain from MCPH1 fused to eGFP. Can be used to detect yH2AX at DNA damage sites.

Depositor

Tuncay Baubec

Article

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

V5

Expression

Mammalian

Mutation

P101S

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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