We narrowed to 6,088 results for: cat.2

1. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB(EXPR-DS-HDAC5_HDAC3)

   Plasmid

   #114399

   Purpose

   For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-GLUT4-7myc-GFP

   Plasmid

   #52872

   Purpose

   detection and measurement of GLUT4 translocation

   Depositor

   Jonathan Bogan

   Article

   Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

   Insert

   GLUT4 (Slc2a4 Rat, with human c-terminus)
   

   Use

   Retroviral

   Tags

   7x-myc (internal) and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. hNFAT2-409:TALE:VP64:KRɸ

   Plasmid

   #160661

   Purpose

   Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   hNFAT2-409:TALE:VP64:KRɸ
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HLTV-hTRIM21

   Plasmid

   #104973

   Purpose

   Purification of His-Lipoyl-hTRIM21 protein from bacteria

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Tags

   His-Lipoyl

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBD-SpyTag-noro-VLP

   Plasmid

   #165989

   Purpose

   Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyTag-noro-VLP (ORF2 Synthetic, norovirus)
   

   Use

   Tags

   SpyTag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB402SC

   Plasmid

   #224552

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Use

   Tags

   Twin-Strep-HA

   Expression

   Plant

   Mutation

   Promoter

   2 X 35S

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB402SN

   Plasmid

   #224551

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Use

   Tags

   HA-Twin-Strep

   Expression

   Plant

   Mutation

   Promoter

   2 X 35S

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS NL4-3 envFS eGFP PR-D25A

   Plasmid

   #101334

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Protease D25A

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCk2_spacer

    Plasmid

    #136072

    Purpose

    Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. GFP-WIPI2d-TEVcs-TSF

    Plasmid

    #171420

    Purpose

    For the purifcation of GFP- WIPI2d protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    WIPI2d (WIPI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF_AfRbcLS

    Plasmid

    #229518

    Purpose

    Expresses Anthoceros fusiformis Rubisco large and small subunit (C terminus hexahistidine)

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco large subunit
    Rubisco small subunit
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF_PcRbcLS

    Plasmid

    #229519

    Purpose

    Expresses Phaeoceros carolinianus Rubisco large and small subunit (C terminus hexahistidine)

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco large subunit
    Rubisco small subunit
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF_NoRbcLS

    Plasmid

    #229520

    Purpose

    Expresses Notothylas orbicularis Rubisco large and small subunit (C terminus hexahistidine)

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco large subunit
    Rubisco small subunit
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSF_PpRbcLS

    Plasmid

    #229521

    Purpose

    Expresses Phymatoceros phymatodes Rubisco large and small subunit (C terminus hexahistidine)

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco large subunit
    Rubisco small subunit
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF_LdRbcLS

    Plasmid

    #229522

    Purpose

    Expresses Leiosporoceros dussi, Rubisco large and small subunit (C terminus hexahistidine)

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco large subunit
    Rubisco small subunit
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_AaRbcLS

    Plasmid

    #229505

    Purpose

    Expresses Anthoceros agrestis Rubisco large and small subunit (C terminus hexahistidine)

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco large subunit
    Rubisco small subunit
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

    Plasmid

    #233868

    Purpose

    Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    (–)-Eldh2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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