Addgene

We narrowed to 19,101 results for: ren

Showing: 521 - 540 of 19101 results

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sgRNA 2 GAPDH

Plasmid

#83809

Purpose

Express Sg-2 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA 1 GAPDH

Plasmid

#83808

Purpose

Express Sg-1 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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2a-copGFP(+HA)

Plasmid

#83806

Purpose

Express copGFP reporter for HDR-induced knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

copGFP

Use

Homologous recombination for gapdh loci

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-peg

Plasmid

#141081

Purpose

Gateway compatible prime editing gRNA empty backbone vector

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-th-sgRNA

Plasmid

#65565

Purpose

in vitro trancription of sgRNA targeting the zebrafish th locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th sgRNA (th Zebrafish)

Use

In vitro rna transcription

Tags

Expression

Mutation

Promoter

T7

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E

Plasmid

#175027

Purpose

Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B L69E

Plasmid

#175026

Purpose

Expresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (L69E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Leucine 69 to Glutamate (CTA to GAA)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEL686

Plasmid

#207510

Purpose

ePPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Insert

zCas9 (H840A)-NC-M-MLV RT-deltaRH

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1A

Plasmid

#198168

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ2

Plasmid

#198173

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-2 μ2

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B K88E

Plasmid

#175028

Purpose

Expresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K88E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 88 to Glutamate (AAG to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B I92E

Plasmid

#175029

Purpose

Expresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (I92E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Isoleucine 92 to Glutamate (ATC to GAA)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B C93E

Plasmid

#175030

Purpose

Expresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (C93E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Cysteine 93 to Glutamate (TGC to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B K128E

Plasmid

#175031

Purpose

Expresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K128E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 128 to Glutamate (AAG to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171835

Purpose

Mammalian expression of human WIPI2d R108E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

R108E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171836

Purpose

Mammalian expression of human WIPI2d R125E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

R125E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171837

Purpose

Mammalian expression of human WIPI2d K128E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

K128E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171842

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep and mCherry

Expression

Mammalian

Mutation

R108E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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