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1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159673

Purpose

Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUAST-FOXO 3A

Plasmid

#52983

Purpose

insect expression of FOXO triple mutant

Depositor

Biao Wang, Marc Montminy

Insert

FOXO (foxo Fly)

Use

Tags

3x HA

Expression

Insect

Mutation

3A, triple mutant, T44A S190A S259A

Promoter

Hsp70

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00638

Plasmid

#39705

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3090 (Sox14 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00474

Plasmid

#39584

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17077 (pnt Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00118

Plasmid

#34272

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7345 (Sox21a Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab35 WT

Plasmid

#37741

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pAc-HT-Dmp53

Plasmid

#85698

Purpose

expression of Drosophila p53 in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Ac5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Use

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pUASTattB-GFP-Msp300KASH

Plasmid

#170806

Purpose

Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

Depositor

Vikki Weake

Article

Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

Insert

GFP-Msp300KASH

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

5xUAS + hsp70 minimal promoter

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-DREDD-V5-His

Plasmid

#63749

Purpose

Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

Depositor

Cyril Gueydan

Insert

Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)

Use

Tags

V5-6XHis

Expression

Insect

Mutation

Promoter

pMT

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab11 DN

Plasmid

#44831

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S25N (dominant negative)

Promoter

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2G09

Plasmid

#70923

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

E(z) (E(z) Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00732

Plasmid

#39749

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3848 (trr Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only
OA-1050S (UASt-dCasRx)

Plasmid

#132419

Purpose

Express dCasRx under UASt promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

CasRx
UASt+hsp70

Use

Tags

Expression

Insect

Mutation

Promoter

UASt

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC+CKK

Plasmid

#59048

Purpose

for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

eGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 534–1457

Plasmid

#59050

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

eGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pTP10

Plasmid

#165797

Purpose

Drosophila TP10 poly(a) terminator element

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

TP10

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDmActin5c

Plasmid

#165777

Purpose

Drosophila Actin 5C promoter

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Actin5c

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-SK(-) Dmel Or22b

Plasmid

#59642

Purpose

Probe for Drosophila melanogaster Or22b expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or22b (Or22b Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00340

Plasmid

#34463

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8573 (su(Hw) Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only