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TRAV1-1

Plasmid

#123367

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV1-1 (TRAV1-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-mpx

Plasmid

#191826

Purpose

mpx probe generation for in situ

Depositor

Jason Berman

Article

Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

Insert

mpx cDNA (mpx Zebrafish)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304-GW-DCK*-IRES-GFP

Plasmid

#176291

Purpose

Gateway vector for use in generating POI-DCK* fusion reporter

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-GW-IRES-GFP

Plasmid

#176289

Purpose

Gateway vector for use in generating DCK*-POI fusion reporter

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-IRES-GFP

Plasmid

#176297

Purpose

Expresses DCK* and GFP from the same transcript

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-IRES-Td

Plasmid

#176298

Purpose

Expresses DCK* and TdTomato from the same transcript

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7/8 subtype NGS Wnt

Plasmid

#159628

Purpose

Express Fzd7/8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Tags

Fc tag and His tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Tags

His-SUMO-TEV

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7 subtype NGS Wnt

Plasmid

#159626

Purpose

Express Fzd7 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-Fzd4 subtype NGS Wnt

Plasmid

#159625

Purpose

Express Fzd4 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd4 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd8 subtype NGS Wnt

Plasmid

#159627

Purpose

Express Fzd8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-WT

Plasmid

#90331

Purpose

expresses Wild type BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7D

Plasmid

#90007

Purpose

expresses 7D mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484D, 488S to 488D, 492S to 492D,…

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7A

Plasmid

#90006

Purpose

expresses 7A mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484A, 488S to 488A, 492S to 492A,…

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA.008

Plasmid

#22841

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA (with MCS)

Use

Cotton vigs vector

Expression

Bacterial

Mutation

Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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