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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Use

Tags

His-SUMO-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Use

Tags

Fc tag and His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7/8 subtype NGS Wnt

Plasmid

#159628

Purpose

Express Fzd7/8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Use

Tags

His6 and bdSUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-Fzd4 subtype NGS Wnt

Plasmid

#159625

Purpose

Express Fzd4 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd4 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7 subtype NGS Wnt

Plasmid

#159626

Purpose

Express Fzd7 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RKS-Fzd8 subtype NGS Wnt

Plasmid

#159627

Purpose

Express Fzd8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-WT

Plasmid

#90331

Purpose

expresses Wild type BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7D

Plasmid

#90007

Purpose

expresses 7D mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484D, 488S to 488D, 492S to 492D,…

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-BRD4-7A

Plasmid

#90006

Purpose

expresses 7A mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484A, 488S to 488A, 492S to 492A,…

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA.008

Plasmid

#22841

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA (with MCS)

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Promoter

Available since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA010

Plasmid

#22843

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing GFP

Use

Cotton expression vector

Tags

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus A component with Coat P…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA009

Plasmid

#22842

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing CHLI fragment

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Src

Plasmid

#19965

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

Src (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

K295M

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Fyn

Plasmid

#16033

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative: K299M

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only