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pSEVA231-CRISPR

Plasmid

#138712

Purpose

Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC myc hPOT1 delta OB

Plasmid

#13241

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 delta OB (POT1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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Geph CR shRNA

Plasmid

#121068

Purpose

shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l4-G'

Plasmid

#209124

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a2-GEMS(JAK-STAT)

Plasmid

#209110

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(JAK-STAT)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G'-IRES-EmGFP

Plasmid

#209127

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G-IRES-EmGFP

Plasmid

#209126

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sizzled HA

Plasmid

#24683

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus chordin myc

Plasmid

#24682

Depositor

Edward De Robertis

Article

Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

Insert

Chordin

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
m2-26 mTRF2

Plasmid

#12300

Depositor

Titia de Lange

Article

Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Insert

mTRF2 (Terf2 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pBluescript SK +/- mTRF1

Plasmid

#12303

Depositor

Titia de Lange

Article

Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

Insert

mTRF1 (Terf1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
EYFP-NLS-actin-K68/284R

Plasmid

#60616

Purpose

Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

K68R, 284R

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
RNF34

Plasmid

#119937

Purpose

RING finger protein 34 expression

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus Frzb-1

Plasmid

#24694

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

Frzb-1 (frzb-1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 xNoggin

Plasmid

#24960

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Noggin (nog.L Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus cerberus

Plasmid

#24679

Depositor

Edward De Robertis

Article

Bouwmeester et al Nature. 1996 Aug 15. 382(6592):595-601.

Insert

Cerberus (cer1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

Plasmid

#175549

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

WAPL (Wapl Mouse)

Use

Mouse Targeting

Tags

AID[71-114]-eGFP

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pX330-NQL005-SOX2-sgRNA

Plasmid

#175553

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 mouse CV2 PC

Plasmid

#25776

Depositor

Edward De Robertis

Article

Coffinier et al Mech Dev. 2002 Dec . 119 Suppl 1():S179-

Insert

cross veinless 2 (Bmper Mouse)

Use

Tags

PC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only

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