We narrowed to 1,336 results for: pET28a

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PET28a-cpLumiLuc-HSA sensor

Plasmid

#186294

Purpose

Expression Human serum albumin sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-HSA sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E143amb)-His

Plasmid

#168046

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 143 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) WT

Plasmid

#108917

Purpose

Express WT Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Tags

His6

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

Plasmid

#117979

Purpose

SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1-B2

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-HIS-ybbr

Plasmid

#117980

Purpose

SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mTagBFP2-TEV-Strep-His

Plasmid

#210824

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mTagBFP2

Tags

TwinStrep, 10xHis

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)_beta2M (MHC Class I light)

Plasmid

#187357

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

beta2M (MHC Class I light)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(40nm)-LPETGG

Plasmid

#90475

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(40nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Tim Mitchison, Jennifer Tirnauer

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-CohIII-CBM-HIS-ybbR

Plasmid

#60866

Purpose

Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

Depositor

Hermann Gaub

Article

Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

Inserts

CohIII
CBM

Tags

6xHIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

Cysteine 63 to Serine in CBM module

Promoter

T7

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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