We narrowed to 1,322 results for: pET28a

1. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-Hook3 D102A, E108A

   Plasmid

   #74618

   Purpose

   Expresses a double mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pet28a His6-HSPB7 (zebrafish) WT

   Plasmid

   #108917

   Purpose

   Express WT Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

   Plasmid

   #117979

   Purpose

   SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_N2N3-B1-B2
   

   Use

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SdrG_N2N3-B1-HIS-ybbr

   Plasmid

   #117980

   Purpose

   SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_N2N3-B1
   

   Use

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(+)_beta2M (MHC Class I light)

   Plasmid

   #187357

   Purpose

   Bacterial expression and refolding of gp100 pMHC

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   beta2M (MHC Class I light)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Use

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Cys-ELP(40nm)-LPETGG

    Plasmid

    #90475

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(40nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-GGG-ELP(40nm)-Cys

    Plasmid

    #91571

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(40nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid

    #60866

    Purpose

    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    CohIII
    CBM
    

    Use

    Tags

    6xHIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Cysteine 63 to Serine in CBM module

    Promoter

    T7

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. human EB1 in PET28a for protein expression

    Plasmid

    #39297

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Article

    Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid

    #60865

    Purpose

    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    Xyl
    Xmod-DocIII
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Threonine 120 to Cysteine in Xylanase domain

    Promoter

    T7

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-mTagBFP2-TEV-Strep-His

    Plasmid

    #210824

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mTagBFP2
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28a-His6-Halo-Tev-Keap1

    Plasmid

    #62456

    Purpose

    His6-halo tagged human Keap1 protein for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+) NRP1 b1b2 (273-586)

    Plasmid

    #162733

    Purpose

    Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1b2 domains (residues 273-586)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Codon optimised

    Promoter

    Available Since

    March 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a His tag-FRB-Venus C

    Plasmid

    #100980

    Purpose

    Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    His-FRB-Venus C
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Cys-ELP(120nm)-LPETGG

    Plasmid

    #90472

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(120nm)-LPETGG
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge