We narrowed to 70,836 results for: LAS

1. pUC19-link-mCherry-FLAG:cat

   Plasmid

   #211831

   Purpose

   Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

   Depositor

   Peng Chen

   Insert

   mCherry-FLAG-cat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-link-PAGFP-FLAG:Kan

   Plasmid

   #211834

   Purpose

   Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAGFP-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV3XFLAGm43CT

   Plasmid

   #17664

   Depositor

   Cecilia Lo

   Insert

   Cx43 CT (Gja1 Mouse)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Cytoplasmic tail: aa 236-382

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4134897

   Plasmid

   #151948

   Purpose

   Mammalian expression plasmid for Human coronavirus HKU1 nucleocapsid

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   Human coronavirus HKU1 nucleocapsid phosphoprotein (N Human coronavirus HKU1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCB52

   Plasmid

   #69747

   Purpose

   Retroviral expression of Cyclin B1

   Depositor

   Iain Cheeseman

   Insert

   Cyclin B1 (CCNB1 Human)
   

   Use

   Retroviral

   Tags

   eGFP-TEV-S

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

6. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

   Plasmid

   #186047

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8f
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

   Plasmid

   #186048

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8m
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p406GALS

   Plasmid

   #17422

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   p406GALS (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALS promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM510

   Plasmid

   #134227

   Purpose

   Retroviral plasmid expressing mCherry - HJURP

   Depositor

   Iain Cheeseman

   Insert

   mCherry-HJURP-LacI 1-304 (HJURP Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid

    #195487

    Purpose

    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    HaloTag.v7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C61T, C262V

    Promoter

    T7

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mT2A-td-sfGFP-FNF

    Plasmid

    #182339

    Purpose

    Recombineering cassette plasmid for T2A-td-sfGFP-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-td-sfGFP-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Z-MATR3-4

    Plasmid

    #166558

    Purpose

    scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST26-his-hSFRP1

    Plasmid

    #189539

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPK719

    Plasmid

    #38149

    Depositor

    Patricia Kuwabara

    Insert

    cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)
    

    Use

    Recombineering

    Tags

    Expression

    Worm

    Mutation

    Promoter

    unc-119

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV Tor1a-Myc

    Plasmid

    #175134

    Purpose

    Lentiviral expression of Myc-tagged mouse Tor1a

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS ZfHoxb1a

    Plasmid

    #27519

    Depositor

    Victoria Prince

    Insert

    Hoxb1a (hoxb1a Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid

    #160587

    Purpose

    Nickase D10A Cas9 for Nt and Ct fusion.

    Depositor

    Diego Orzaez

    Insert

    nickase D10A Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid

    #102771

    Purpose

    "Activate neurons with 2p stimulation"

    Depositor

    Karel Svoboda

    Insert

    C1V1::FusionRed::Kv2.1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1 traficking domain

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.3_HES2

    Plasmid

    #124070

    Purpose

    HES2 with normal stop codon

    Depositor

    J. Chad Brenner

    Insert

    HES2 (HES2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. SPIN3A

    Plasmid

    #74656

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN3A (SPIN3 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

22. A-WDR5-1

    Plasmid

    #166515

    Purpose

    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBABE.puro/TO/Flag/mKO2

    Plasmid

    #110353

    Purpose

    Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag

    Depositor

    Sandra Martha Gomes Dias

    Insert

    monomeric Kusabira Orange 2
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    gag

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBS ZfHoxa2b

    Plasmid

    #27514

    Depositor

    Victoria Prince

    Insert

    Hoxa2b (hoxa2b Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

25. EGFP-hPMCA4bct125

    Plasmid

    #47594

    Depositor

    Emanuel Strehler

    Insert

    PMCA4bct125 (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    125 C terminal AA deleted

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

26. PP-SRM

    Plasmid

    #25110

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PF11_0301:C22904 (PF11_0301 Plasmodium falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

27. PP-ClpR

    Plasmid

    #32876

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ClpR (PF3D7_1436800 Plasmodium Falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

28. psicheck_NDUFA6_3UTR

    Plasmid

    #65886

    Purpose

    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR

    Depositor

    Thomas Tuschl

    Insert

    NDUFA6 (NDUFA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pBS ZfHoxb2a

    Plasmid

    #27521

    Depositor

    Victoria Prince

    Insert

    Hoxb2a (hoxb2a Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

30. Py-PPI (PY00382)

    Plasmid

    #25567

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Py-PPI (PY00382) (PY00382 Plasmodium yoelii)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits