We narrowed to 13,181 results for: OVA

1. pBLO 62.4

   Plasmid

   #123123

   Purpose

   Human expression vector for deltaproteobacteria CasX and sgRNA

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

   Plasmid

   #69534

   Purpose

   DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   DD-dCas9VP192-T2A-EGFP
   

   Use

   Tags

   C-term T2A-EGFP and DHFR Destabilised Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. L-GARS-2

   Plasmid

   #166531

   Purpose

   scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBLO 62.5

   Plasmid

   #123124

   Purpose

   Human expression vector for planctomyces CasX and sgRNA

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Inserts

   CasX protein
   CasX sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDest27-mBIN1+13+17

   Plasmid

   #61097

   Purpose

   Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

6. psigma1-EGFP

   Plasmid

   #53611

   Purpose

   Expresses sigma1 of adaptor protein of AP1

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   sigma 1 (Ap1s1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUW-TetO-MXD1

   Plasmid

   #178443

   Purpose

   doxycycline-inducible expression of Human MXD1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   MXD1 (MXD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-Lyl1

   Plasmid

   #131612

   Purpose

   expression of mouse LyI1 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Lyl1 (Lyl1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
    

    Use

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-eGFP-NMHC-IIAΔtailpiece

    Plasmid

    #35689

    Depositor

    Tom Egelhoff

    Article

    Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

    Insert

    MHC IIA (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Added stop codon after codon 1928, eliminating th…

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T3-GST-EGFP

    Plasmid

    #177136

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAIP-hXRCC1pd-eGFP

    Plasmid

    #206033

    Purpose

    Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    CAG

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-VPS35

    Plasmid

    #21689

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

    Plasmid

    #176587

    Purpose

    Bacterial expression of His14-MBP-TEV-AmaTnpB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Use

    Tags

    His14 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330.Pten.b

    Plasmid

    #66588

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. p3xFLAG-CMV-Tlr4

    Plasmid

    #27148

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4 (Tlr4 Mouse)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Native signal peptide sequence (original amino ac…

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAAV.CAG.Flex.Twitch2B.WPRE.SV40

    Plasmid

    #100039

    Purpose

    AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch2B
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NLS-CanlonicSF

    Plasmid

    #188390

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    NLS-CanlonicSF
    

    Use

    Tags

    NLS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pyc111f

    Plasmid

    #158780

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6f-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBabe-puro/HA-FLAG-RBP2

    Plasmid

    #14802

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHS0806 His14-bdSUMO-AwaIscB

    Plasmid

    #176539

    Purpose

    Bacterial expression of His14-bdSUMO-AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Use

    CRISPR

    Tags

    14xHis-bdSUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RET-V5

    Plasmid

    #139627

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Twitch-3 pcDNA3

    Plasmid

    #49532

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-3
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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