...Institute for Protein Innovation Antibody Collection Institute for Protein Innovation Antibody Collection...Plasmids Welcome to the Institute for Protein Innovation (IPI) Collection! Here, you’ll find unique recombinant...

...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...We should continue to examine our behavior and innovate solutions and practices to meet higher environmental...

..., we need to take sex into account. Gendered Innovations is an international, collaborative project—funded...creative power of sex and gender analysis for innovation and discovery. Tackling sex and gender in research...organizations. “Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ...invested in the trial). The goal of gendered innovations It is crucially important to get the research... the beginning. This is the goal of Gendered Innovations. This project: 1) develops state-of-the-art methods...of how gender analysis leads to discovery and innovation. One such case study looks at the effects of ...

... the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in synthetic ...

...MUST be placed in an ice bucket immediately after removal from the -20 °C freezer because heat can cause ...Polymerase or Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt...

...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA ...enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join...

...control both when and where genome editing happens. CASANOVA , developed by Dominik Niopek’s lab , is a way...improved kinetics LSD1 - repression through targeted removal of H3K4 and H3K9 methylation In certain cases, ... maintained by the Jennifer Doudna lab at the Innovative Genomics Institute, University of California,... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, ...Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli, C., & Concordet, J. P. (2018). CtIP fusion ..., Ladha, A., Gardner, Z., Schmid-Burgk, J. L., Makarova, K. S., Koonin, E. V., & Zhang, F. (2019). RNA-guided...

...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that...facilitation for the transfer plasmid, including legal approval and an implementation letter DNA amplification...business days! Ordering At your convenience After approval, the next step is yours: you must place your order...before we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization...

...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...CRISPRainbow. Nat Biotechnol . . PMID: 27088723 Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. 2015. In vivo... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...26422227 Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury...

...A, Sanchez REA, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shulga L, Sigler AR, Siverts LA, Somasundaram...Radaelli C, Gore BB, Weed N, Omstead V, Bishaw Y, Shapovalova NV, Martinez RA, Fong O, Yao S, Mortrud M, Chong...

...members from industry and academia financed by the Innovative Medicine Initiative (IMI) of the European Union...RESOLUTE project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under...the European Union's Horizon 2020 research and innovation programme and EFPIA. Figure 1: Representation...

... viral service. Retrograde construct from Alla Karpova at Janelia Research Institute....preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector... these viral preparations was performed by the Karpova lab. Results for our quality control experiments...

...85040 pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet...PI 11578 pSico Cre activates shRNA expression, removal of EGFP; See also similar plasmids pSico PGK GFP...Jacks 11579 pSicoR Cre turns off shRNA expression, removal of EGFP; See also similar plasmids pSicoR PGK puro...

...and two-way ANOVAs ANOVA tests can compare either one (one-way ANOVA) or two (two-way ANOVA) independent...different within your ANOVA/ANCOVA analysis. If you have performed a MANOVA/MANCOVA and want this information... form of ANOVA. Isn’t that what a two-way ANOVA test is you might ask? Not exactly! A MANOVA test assesses...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...antagonistically, were additive, etc.). ANCOVA and MANCOVA All variants of ANOVAs discussed so far assume that the...each other – then a MANCOVA or an ANCOVA can measure this effect, known as covariance. This is the “C” that...differentiates these tests from ANOVA/MANOVAs. Quantifying and controlling for any covariance between manipulated ...

...Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will be indicated...antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody preparations...

... post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...quantum yield and the extinction coefficient) (Shcherbakova et al., 2015). The effective brightness reflects...is perfect for use in spectral multiplexing (Shcherbakova et al., 2018). NIR FPs can be combined with ...three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as in four-color imaging... degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For detection of protein-protein...recommend using NIR split reporters based on miRFPs (Shcherbakova et al., 2016), since they have lower background...miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and iGECI calcium indicator (Shemetov...

....2023.12.034 Kolesnik, M. V., Fedorova, I., Karneyeva, K. A., Artamonova, D. N., & Severinov, K. V. (2021...0299-x Moya-Beltrán, A., Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A.,..., F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E. V., & Zhang,...doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014...doi.org/10.1134/s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas.../doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., & Zhang, F. (2017). Diversity, classification...–78. https://doi.org/10.1016/j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

...’s lab in Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc...research. When she came to Novartis, she was happily surprised that “Novartis was actually really research...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new tool that allows ... and says that she decided to do a post-doc at Novartis because she wanted first-hand experience in industry...investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ...developing more potent ABL1 allosteric inhibitors at Novartis. The ClonTracer library has been distributed all...