We narrowed to 7 results for: OVA

1. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...MUST be placed in an ice bucket immediately after removal from the -20 °C freezer because heat can cause ...Polymerase or Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt...

2. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA ...enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join...

3. General Transfection

   Type

   Protocol

   ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

4. Gibson Assembly Protocol

   Type

   Protocol

   ... the annealed DNA fragments. Taq DNA Ligase - covalently joins the annealed complementary DNA fragments...

5. Lentivirus Production

   Type

   Protocol

   ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

6. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ... ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount of background...

7. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...Functional siRNAs and miRNAs exhibit strand bias . Khvorova A et. al. Cell . 2003. 115:209-216. PubMed . A...