...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...We should continue to examine our behavior and innovate solutions and practices to meet higher environmental...

..., we need to take sex into account. Gendered Innovations is an international, collaborative project—funded...creative power of sex and gender analysis for innovation and discovery. Tackling sex and gender in research...organizations. “Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ...invested in the trial). The goal of gendered innovations It is crucially important to get the research... the beginning. This is the goal of Gendered Innovations. This project: 1) develops state-of-the-art methods...of how gender analysis leads to discovery and innovation. One such case study looks at the effects of ...

... the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in synthetic ...

...and two-way ANOVAs ANOVA tests can compare either one (one-way ANOVA) or two (two-way ANOVA) independent...different within your ANOVA/ANCOVA analysis. If you have performed a MANOVA/MANCOVA and want this information... form of ANOVA. Isn’t that what a two-way ANOVA test is you might ask? Not exactly! A MANOVA test assesses...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...antagonistically, were additive, etc.). ANCOVA and MANCOVA All variants of ANOVAs discussed so far assume that the...each other – then a MANCOVA or an ANCOVA can measure this effect, known as covariance. This is the “C” that...differentiates these tests from ANOVA/MANOVAs. Quantifying and controlling for any covariance between manipulated ...

... post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...quantum yield and the extinction coefficient) (Shcherbakova et al., 2015). The effective brightness reflects...is perfect for use in spectral multiplexing (Shcherbakova et al., 2018). NIR FPs can be combined with ...three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as in four-color imaging... degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For detection of protein-protein...recommend using NIR split reporters based on miRFPs (Shcherbakova et al., 2016), since they have lower background...miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and iGECI calcium indicator (Shemetov...

....2023.12.034 Kolesnik, M. V., Fedorova, I., Karneyeva, K. A., Artamonova, D. N., & Severinov, K. V. (2021...0299-x Moya-Beltrán, A., Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A.,..., F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E. V., & Zhang,...doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014...doi.org/10.1134/s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas.../doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., & Zhang, F. (2017). Diversity, classification...–78. https://doi.org/10.1016/j.mib.2017.05.008 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

...’s lab in Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc...research. When she came to Novartis, she was happily surprised that “Novartis was actually really research...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new tool that allows ... and says that she decided to do a post-doc at Novartis because she wanted first-hand experience in industry...investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ...developing more potent ABL1 allosteric inhibitors at Novartis. The ClonTracer library has been distributed all...

...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland.... Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting...Crystal's educational background and position at Novartis. Chapter 2: What's a lab group in industry? The...empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself for success by having... The types of science that Crystal works on at Novartis, how she's learned to prioritize projects, and...listings. Mobility between different positions at Novartis. Chapter 7: How Crystal developed her science ...communication skills and how they've helped her at Novartis. The importance of mentorship in developing career...

...✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation that may optionally...repair system, and more recent prime editing innovations have improved the odds of mismatch repair favoring... C-terminus of Cas9 H840A nickase (PE1). But innovations by the Liu lab and others have led to a variety...cells with 1-10% indels. Since then, further innovations have continued to improve the maximum efficiency...expression and activity In addition to the numerous innovations that streamline the process of prime editing,...reduced editing efficiencies, likely because both innovations aim to solve the same problem. Both approaches... this table helps you keep them straight! Innovation Purpose PE2 PE3 PE4 PE5 PEmax PE6...

...catalytic activity and instead become trapped in a covalent bond with its substrate. Then, all it takes is...on the human DNA repair protein AGT that gets covalently linked to derivatives of its substrate O6-benzylguanine... 2005). Although this tight interaction is not covalent, later work showed that introducing a Leu28Cys...Leu28Cys mutation in eDHFR does lead to covalent binding of the TMP-tag ligand (Gallagher et al., 2009). HaloTag...substrate-linked PEG, biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate...Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo Covalent TMP-Tag Based on Proximity-Induced Reactivity.... Adams, S. R., & Tsien, R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside...

...However, if we think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable...ll delve into strategies you can use to foster innovation. If you identify as a non-creative or if you ... by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself a block of...As a result, the product team is an incredibly innovative group. Our entire company benefits from their... about the future will improve your ability to innovate. (Finkelstein, 2019) One issue currently plaguing... S. (2019, July 15). A brief exercise to spur innovation on your team. Harvard Business Review. Retrieved...https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-your-team   Additional Resources on the Addgene...

...AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating the CASANOVA complex,...Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to ...their most potent Acr-LOV fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel...observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector...genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods...

... by our partners at the Institute for Protein Innovation (IPI), part of a new collection of epitope tag...Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). This construct contains the Lon...substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license. This construct...amenable to a wide range of biophysical assays (Kroupova et al., 2024). They were able to study and crystallize...degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of a Cereblon construct...

...Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander....054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

...ligase to covalently link the fragments together at the expense of ATP (see below, covalent bonds are ...restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby... to make bonds between the insert and backbone covalent.  Before beginning the restriction digest...orientation. You then add ligase to the mixture to covalently link the backbone and insert and, PRESTO, you...

...mycoplasma removal from the infected culture. MRAs are non-toxic, effective in the removal of most of ...Started with Tissue Culture below! Mycoplasma removal after detection So you found mycoplasma contamination...contamination to other healthy cell lines. Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family...mycoplasma. These methods cannot guarantee complete removal of mycoplasma but, can get rid of most of mycoplasma...

...of DNA transposon transposition. Modified from Sandoval-Villegas et al., 2021. To mobilize a transposon...inactivated Tc1/mariner transposons found in fish (Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred...or negative selected for during the experiment (Sandoval-Villegas et al., 2021). Target site preference... including zebrafish, mice, rats, and rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons...topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021...

...our partnership with the Institute for Protein Innovation (IPI), we expanded a growing collection of thoroughly...enter 2025, your feedback continues to guide our innovation.  In response to a demand for more AAV viral ...extensive plasmid database — because we believe that innovation flourishes when data are shared. The scientific...look ahead, I am excited to continue to grow and innovate with your help and guidance. Every plasmid shared...