We narrowed to 6,002 results for: ATS

1. pCG-NT2

   Plasmid

   #229848

   Purpose

   Expresses wild-type Cas9 and gRNA with non-target sequence.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA with non-target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hPhyB621(Y276H)-P2A-m1V

   Plasmid

   #197377

   Purpose

   The AAV vector for PhyB621(Y276H) and m1V

   Depositor

   Michiyuki Matsuda

   Insert

   Phytochrome B Y276H(1-621 aa)
   

   Use

   AAV

   Tags

   P2A-m1V

   Expression

   Mutation

   Human codon optimized PhyB (1-621 aa), Y276H muta…

   Promoter

   CMV

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABEmax-SpRY

   Plasmid

   #195279

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

   Depositor

   Petros Patsali, Carsten Lederer

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

   Plasmid

   #110547

   Purpose

   CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

   Depositor

   Ichiro Matsumura

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pBAD

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABEmax-SpG

   Plasmid

   #195278

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

   Depositor

   Petros Patsali, Carsten Lederer

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-9-RTXWT-C

   Plasmid

   #225964

   Purpose

   Block V Repeats-In-Toxin (RTX) protein with C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXWT-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2/shαSMA/GFP4_Seq1/clone1

   Plasmid

   #206138

   Purpose

   Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Insert

   alpha-SMA (Acta2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-Puro_hCOL4A4-Myc-His

   Plasmid

   #198093

   Purpose

   Express hCOL4A4 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A4 (COL4A4 Human)
   

   Use

   Tags

   TEV-Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRGFP

   Plasmid

   #26475

   Purpose

   In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

   Depositor

   Maria Jasin

   Article

   Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

   Insert

   DRGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. Venus-pBAD

    Plasmid

    #54859

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. mKikGR-C1

    Plasmid

    #54656

    Purpose

    Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Habuchi et al PLoS One. 2008;3(12):e3944. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    mKikGR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28CLY1

    Plasmid

    #21760

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible one SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-Rex

    Plasmid

    #122134

    Purpose

    B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    B. subtilis Rex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    apFAB338

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROP-IP-GFP

    Plasmid

    #122136

    Purpose

    GFP Reporter plasmid for B-Rex contain imperfect operator sequence

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28CLY3

    Plasmid

    #21762

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pET28CLY2

    Plasmid

    #21761

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing two SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

17. mVenus-N1

    Plasmid

    #54640

    Purpose

    Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. mVenus-C1

    Plasmid

    #54651

    Purpose

    Localization: C1 Cloning Vector, Excitation: 515, Emission: 528

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET28CLY9

    Plasmid

    #21769

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pSB207

    Plasmid

    #164122

    Purpose

    J23101 driving PP7 repeats

    Depositor

    Christopher Voigt

    Article

    Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

    Insert

    YFP-PP7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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