...a group photo wearing holiday sweaters and Santa hats. There was even a giant taco... Miller lab annual...

...among the most important are the inverted terminal repeats (ITRs). ITRs are the elements that make an AAV ...guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch...2019). A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Hum Gene Ther Methods...the B-B’ and C-C’ regions of inverted terminal repeats reduces rAAV productivity but increases transgene...

... from that of wild cats with the goal of optimizing the health of domesticated cats and learning about...data for analysis. And, well, cats... everyone knows the internet loves cats! This is mostly a project to...

...Further, the pools are available in two vector formats: all-in-one (lentiCRISPRv2, expressing both Cas9...biological insight. Pooled and individual plasmid formats A first for a library at Addgene, you can also ...

...lab For more information about limitations and caveats of Cre usage: Vagaries of conditional gene targeting...

...that’s on it are plants, and the animals you eat ate plants too. Think about how many people are starving...

...consisting of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of...of ECFP and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and...and EYFP connected by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected... connected by 5 GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected...connected by 6 GGSGGS repeats pET28CLY7 Peptide linker standard consisting of ECFP and EYFP connected by 7 GGSGGS...GGSGGS repeats pET28CLY8 Peptide linker standard consisting of ECFP and EYFP connected by 8 GGSGGS repeats...consisting of ECFP and EYFP connected by 9 GGSGGS repeats pmVenus(L68V)-mTurquoise2 Brightness standard used...

... domain is composed of 33-35 amino acid repeats. These repeats only differ from each other by two amino... of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the...from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of...

... customizable arrays of polymorphic amino acid repeats in the TAL effectors. View Addgene's TALEN Guide...Plasmid 47389 also contains the VP64 domain (four repeats of the minimal activation domain of VP16) at the...cell expression, and encode one of three 0.5 TALE repeats (C, T, and G) and the full length human LSD1 at...

...clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be...s protospacer sequence and PAM fall on the top (Watson) strand ( Figure 2A ). However, DSB will occur ...by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 in mammalian cells. Canver...

...lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense...knockout screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9...efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid...

... mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE...AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons...

...MGC102867 SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ... semF SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ...

...endothelial cells (mice, rats); CNS neurons (infant rhesus macaques) Mice, rats, rhesus macaques No ... Mice, rats No (mechanism unknown) Krolak et al., 2022 AAV.MaCPNS1 PNS (mice and rats) CNS; ...mice) PNS (rats) CNS; PNS, including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets... not effective in NHPs (Liguore et al., 2019; Matsuzaki et al., 2018; Huang et al., 2023). However, several... mouse strains. **AAV1-X1 has not been tested in rats. For more information on reagents and protocols,... Reference AAV-PHP.B* CNS (broad) Mice, rats Yes (LY6A) Deverman et al., 2016 Hordeaux et...for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al., 2017 Brown et al, 2021...

...Zebrafish ( Sander et al., Nat Biotechnol. 2011 ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic...

...recommend or require authors to use specific PID formats. For example, several journals like eLife, Nature...are supported by SciCrunch. Other well known PID formats are resolvable compact identifiers, which are Compact...identifier for every plasmid. In case these PID formats are new to you, we’ll go through examples to highlight.... Despite their differences, both of these PID formats for lenti-Cas9-VQR contain the Addgene ID # and...Addgene. How to cite options using the different PID formats The instructions for how to cite any Addgene plasmid...citing with resolvable compact identifier and RRID formats. Let’s go back to our example plasmid, lenti-Cas9...happy to support the RRID and compact identifier formats. Acknowledgments Many thanks to Daniela Bourges...

...lustered R egularly I nterspaced S hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform...

..., and genetic similarities with humans. However, rats have some key differences in physiology, intelligence...edge as a disease mode (Iannaccone et al., 2009). Rats are larger than mice making them more useful for...research (Ellenbroek and Youn, 2016). For these reasons rats were once the most used mammalian model in medical... perfect. One of the most limiting attributes of rats is their difficult to manipulate genome and limited... have vastly improved scientists’ ability to use rats as disease models.  Check out Addgene’s collection...nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models & Mechanisms, 2(5–6), 206–210. https...

...parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats) on Dec 19, 2018 at 4:36am PST Buddy..., 2018 Eppi the red nosed reindeer from @ladiesinlabcoats Many teams recycled lab materials into holiday...up about sustainability in the lab!). The @ladiesinlabcoats used a pipette tip box and microcentrifuge...love your Insta!! #LILCChristmas . . #lab #ladiesinlabcoats #Christmas #labchristmas #cute #reindeer #...

...meshwork following intracameral injection in mice, rats, and dogs. This serotype was derived from AAV2 capsid...