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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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4E1H.95_LUMABS

Plasmid

#155208

Purpose

Expresses 4E1H.95_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLz33_CsoS2_NTD_GFP

Plasmid

#140861

Purpose

His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD fused to GFP

Tags

Hexahistidine tag and Superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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p415:iCas9

Plasmid

#138266

Purpose

Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mTN3-GGS6-dCas9

Use

CRISPR

Expression

Yeast

Mutation

Cas9-D10A, H840A

Promoter

GalL

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hsg(NT:NT)

Plasmid

#138260

Purpose

Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-FeGFP

Plasmid

#138261

Purpose

Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

eGFP

Use

CRISPR

Expression

Mammalian

Promoter

None

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(∆77-91)

Plasmid

#121516

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 77-91

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T+1X

Plasmid

#108188

Purpose

PCNA1-PdX fusion protein

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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FH95pUG+p97W (B8)

Plasmid

#74017

Purpose

lentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRAF-MutL(R95F)

Plasmid

#86608

Purpose

E. coli Mutl(R95F) expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL(R95F)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

R95F

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-TZAPZNF1-8

Plasmid

#87181

Purpose

retroviral stable expression of truncation protein Flag-TZAPZNF1-8

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-TZAPZNF7-9

Plasmid

#87183

Purpose

retroviral stable expression of truncation protein Flag-TZAPZNF7-9

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pPD1R

Plasmid

#86969

Purpose

Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH

Promoter

T7 promoter

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-TZAPZNF7-11

Plasmid

#87182

Purpose

retroviral stable expression of truncation protein Flag-TZAPZNF7-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-H1-sgRNA-mTZAP

Plasmid

#87187

Purpose

guideRNA targeting exon2 of mouse TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (Zbtb48 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

H1

Available Since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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p1G108CP20X

Plasmid

#85096

Purpose

Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to putidaredoxin

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch5_155183064-gRNA-rev

Plasmid

#81215

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-for2

Plasmid

#81209

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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FH95pUp97aGW (C3)

Plasmid

#74019

Purpose

lentiviral expression of Psd95 shRNA and SAP97alpha-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS2

Plasmid

#69234

Purpose

U6 driven SpCas9 sgRNA expression for TS2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS3

Plasmid

#69235

Purpose

U6 driven SpCas9 sgRNA expression for TS3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS4

Plasmid

#69236

Purpose

U6 driven SpCas9 sgRNA expression for TS4 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_227_Venus_195

Plasmid

#39836

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only