We narrowed to 22,283 results for: fus

1. pGAD-Atg17(∆279-295)

   Plasmid

   #121522

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted amino acids 279-295

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAD-Atg17(∆129-177)

   Plasmid

   #121518

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted amino acids 129-177

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAD-Atg17(∆266-280)

   Plasmid

   #121520

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted amino acids 266-280

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGAD-Atg17(∆15-106)

   Plasmid

   #121515

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted amino acids 15-106

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAD-Atg17(∆186-236)

   Plasmid

   #121519

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted amino acids 186-236

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAD-Atg17(∆266-312)

   Plasmid

   #121521

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted amino acids 266-312

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b+R3(R112C/T180C)C

   Plasmid

   #108194

   Purpose

   Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b+3(R112C/T180C)C

   Plasmid

   #108193

   Purpose

   Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b+2(L171C)X

   Plasmid

   #108192

   Purpose

   Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA2(L171C)-PdX fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   L171C (PCNA2); C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b+R2(L171C)X

    Plasmid

    #108191

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCML 313

    Plasmid

    #98586

    Purpose

    pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC3R112C/T180C

    Plasmid

    #85088

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3

    Promoter

    T7 promoter

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL_Ch5_155183064-155183141

    Plasmid

    #81211

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 5 (numbers in common name refer to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTCW018

    Plasmid

    #83531

    Purpose

    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pARO9/pFUS1J2

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO1-puro-U6-sgRNA-PLXNB2

    Plasmid

    #69240

    Purpose

    U6 driven SpCas9 sgRNA expression for PLXNB2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    PLXNB2 sgRNA (PLXNB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

    Plasmid

    #69221

    Purpose

    Expresses R1333K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

    Plasmid

    #69222

    Purpose

    Expresses R1333S mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1333 is changed to Serine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNB786

    Plasmid

    #60151

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNB682

    Plasmid

    #60143

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSCV-puro-mMCL1 Delta C22

    Plasmid

    #45820

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    mMCL-1 Delta C22
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal 22 amino acids are deleted from full l…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pMSCV-puro-Delta N67-mMCL1

    Plasmid

    #45819

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    Delta N67-mMCL-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N-terminus 67 amino acids deleted from full lengt…

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pFSW Syt1-C2B V304A,I367A IRES GFP

    Plasmid

    #133821

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    V304A, I367A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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26. pFSW Syt1-C2B D363,365N IRES GFP

    Plasmid

    #133822

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D363,365N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFSW Syt1-C2A K189-192A IRES GFP

    Plasmid

    #133823

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    K189-192A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFSW Syt1-C2A D230,232N IRES GFP

    Plasmid

    #133824

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D230,232N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFSW Syt1-C2A M173,F234A IRES GFP

    Plasmid

    #133825

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    M173, F234A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Use

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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