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pAV0765

Plasmid

#133501

Purpose

Expression of mCherry targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX

Expression

Yeast

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Publication

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-EFS:GFP

Plasmid

#68484

Purpose

Retrovirus expressing GFP driven by EFS promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

EFS and LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHBFD1Bad

Plasmid

#53564

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

araBAD

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-PM mir shLEF1 b

Plasmid

#27018

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 1b

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pDP085

Plasmid

#198425

Purpose

pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

Depositor

Markus Schmid

Publication

You et al Nat Commun. 2017 May 17;8:15120. doi: 10

Insert

E. coli BirA

Expression

Plant

Promoter

pUBQ10

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQAT05

Plasmid

#223377

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Publication

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTF101.3

Plasmid

#134772

Purpose

A binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Publication

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.G

Plasmid

#200999

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Publication

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Expression

Yeast

Promoter

CYC1

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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SL-SunTag

Plasmid

#196919

Purpose

The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEPIC1.1

Plasmid

#169897

Purpose

Empty constitutive expression vector with piggyBac, IRES, and mClover3

Depositor

Richard Schneider

Publication

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

3x FLAG 2x StrepII

Expression

Mammalian

Promoter

mouse PGK

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Publication

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Expression

Mammalian

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-15b-PTS

Plasmid

#108953

Purpose

heterologous expression of PTS of P. cablin in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Patchoulol synthase (PTS)

Tags

His6-Tag

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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UKNP4.1.1

Plasmid

#98266

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAB045a

Plasmid

#79224

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ rpoZ-HA4; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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Measles V-FLAG

Plasmid

#44909

Depositor

Curt Horvath

Publication

Palosaari et al J Virol. 2003 Jul;77(13):7635-44.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MeV (P/V/C )

Tags

FLAG

Available since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-KHK

Plasmid

#23586

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KHK (KHK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FN3K

Plasmid

#23621

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FN3K (FN3K Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Expression

Mammalian

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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