We narrowed to 14,493 results for: NTS

Showing: 8161 - 8190 of 14493 results

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pET-Duet1-His-TEV-mCherry-OPTN WT

Plasmid

#190191

Purpose

Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Tags

6xHis, TEV, and mCherry

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 P.1

Plasmid

#173476

Purpose

Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 Spike Truncated to remove 18 amino acids

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p420-3

Plasmid

#71257

Purpose

luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

Depositor

Peter Cockerill

Article

Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

Insert

3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF minimal

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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mH2A2-CT-GFP

Plasmid

#45171

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
PET-B-evoSpCas9-NLS-6xHis

Plasmid

#207390

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-T308A/S473A

Plasmid

#49191

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308A; S473A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194378

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3CC-FLAG

Plasmid

#179162

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-A11(89-196)

Plasmid

#196215

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-68)-Strep

Plasmid

#196216

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-52)-Strep

Plasmid

#196213

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-HA-mCherry-pA

Plasmid

#233030

Purpose

To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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1_T7-mScarlet3-Hras

Plasmid

#225928

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-HRas

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD10

Plasmid

#195848

Purpose

Expresses human FZD10 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD10 (FZD10 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

Plasmid

#139086

Purpose

Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCD565

Plasmid

#153027

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B57

Plasmid

#135516

Purpose

Mammalian expression of myc-tagged HLA-B*57:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*57:1 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ262B-ABE8e

Plasmid

#161524

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

TadA8e-zSpRYCas9(D10A)

Use

CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

Expression

Plant

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mPpp3cc-FLAG-6XHIS

Plasmid

#179160

Purpose

Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)

Tags

6XHIS and FLAG

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139D-GS3

Plasmid

#204748

Purpose

Expression of Cas9 and human H2AX with the S139D mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139D (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139D mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ266E

Plasmid

#161521

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

zSpRYCas9(D10A)-PmCDA1-UGI

Use

CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

Tags

3X FLAG, NLS

Expression

Plant

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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2_T7-mScarlet3-KRas

Plasmid

#225929

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-KRas

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-flex-Clover

Plasmid

#135177

Purpose

AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

Inserts

Clover
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of GFP

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD P35S (GB0552)

Plasmid

#160554

Purpose

35s promoter for N-terminal fusions

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Nucleic Acids Res. 2017 Feb 28;45(4):219

Insert

35s

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBB HA CCDC22

Plasmid

#74903

Purpose

Express CCDC22 in mammalian cells

Depositor

Ezra Burstein

Article

Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

Insert

CCDC22 (CCDC22 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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(364) SB KRAB-SadCas9

Plasmid

#163022

Purpose

Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

Transposon

Tags

KRAB and myc NLS

Expression

Mammalian

Promoter

TET-On

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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