We narrowed to 9,135 results for: Pol

1. CaV1.3e[8a,11,31b,Δ32,42a] mut

   Plasmid

   #26576

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-GGG-HIS-CBM-Xmod-DocIII

   Plasmid

   #91698

   Purpose

   CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-HIS-CBM-Xmod-DocIII
   

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDule2-IBBN (G2)

   Plasmid

   #85501

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBa.TfR-Halo

   Plasmid

   #162716

   Purpose

   Mammalian expression of human TFRC

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   TfR (TFRC Human) (TFRC Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta actin

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBAD_Ub11TCA

   Plasmid

   #174517

   Purpose

   Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   Ubiquitin
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Recoded with TCA replacing codon 11 of wt ubiquit…

   Promoter

   araBAD

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLNCX1 HA Akt1

   Plasmid

   #15990

   Depositor

   Morris Birnbaum

   Article

   Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pRK5 GFP RagB Q99L

   Plasmid

   #112749

   Purpose

   express GFP-RagB (Q99L mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   GFP-RagB (Q99L) (RRAGB Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q99L

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiV_Neo_Sik3_T142Q

   Plasmid

   #119973

   Purpose

   Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Sik3 (Sik3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   change Threonine 142 to Glutamine

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-6P1-Ataxin3Q22

    Plasmid

    #22117

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDF11-SFPQ(276-598)

    Plasmid

    #135437

    Purpose

    Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Splicing Factor Proline And Glutamine Rich (SFPQ Human)
    

    Tags

    hexahistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G03 BATF3

    Plasmid

    #67050

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BATF3 (BATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

    Plasmid

    #221080

    Purpose

    SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3XFlag-Mettl4

    Plasmid

    #86665

    Purpose

    Expresses murine Mettl4 containing N-terminal 3XFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Mettl4 (Mettl4 Mouse)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYLL204

    Plasmid

    #53455

    Purpose

    Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

    Depositor

    Leroy Liu, Lisa Lyu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y814F

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

18. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEMHE-H2B-mClover3

    Plasmid

    #171489

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H2B (H2BC11 Human)
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIS1 DICER1 long UTR

    Plasmid

    #21649

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 long UTR (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge