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pnEA-NpM-CeMextli_471-507opt-M493D-GB1_AB

Plasmid

#148353

Purpose

Bacterial Expression of CeMextli_471-507opt-M493D

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-M493D (Y18D10A.8 Nematode)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-CeMextli_471-507opt-Y479AL484AM485A-GB1_AB

Plasmid

#148354

Purpose

Bacterial Expression of CeMextli_471-507opt-Y479AL484AM485A

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-Y479AL484AM485A (Y18D10A.8 Nematode)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R

Plasmid

#147407

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Publication

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MDH1-pre-let-7_PShairpin

Plasmid

#26537

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

pre-let-7_PS hairpin

Use

Retroviral

Expression

Mammalian

Mutation

5 nt mutated to make pre-let-7 stem pairing perfe…

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPA-IRES-GFP

Plasmid

#26547

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPA

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant A

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPB-IRES-GFP

Plasmid

#26548

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPB

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant B

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPC-IRES-GFP

Plasmid

#26549

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPC

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant C

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPD-IRES-GFP

Plasmid

#26551

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPD

Use

Retroviral

Expression

Mammalian

Mutation

loop mutant D

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Publication

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut_R

Plasmid

#147397

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut

Depositor

Elisa Izaurralde

Publication

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-CIM2mut (TNRC6C Human, Nematode)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_wt-IRES-GFP

Plasmid

#26491

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_2A-IRES-GFP

Plasmid

#26545

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Expression

Mammalian

Mutation

SD1 G->A

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Publication

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159794

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

Plasmid

#107739

Purpose

mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein(C1A)

Expression

Worm

Mutation

change the first cysteine of gp41-1 N-intein to a…

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-50

Plasmid

#46740

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-50

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-lsy-6

Plasmid

#46738

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lsy-6

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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