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pPN255

Plasmid

#91584

Purpose

Express sgRNA targeting human CNOT1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CNOT1 (CNOT1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN170

Plasmid

#91599

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN171

Plasmid

#91600

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN190

Plasmid

#91577

Purpose

Express sgRNA targeting human BRINP2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BRINP2 (FAM5B Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.2.mKO2

Plasmid

#85210

Purpose

TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK5 CBM-E

Plasmid

#53169

Purpose

Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
FNE2-A72

Plasmid

#44608

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2-A72 (Ets2 Mouse)

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

THR-72 converted to Alanine

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+MLS-HyPer7

Plasmid

#136470

Purpose

Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Tandem mitochondrial targeting signal of cytochro…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-NLS-HA-TurboID

Plasmid

#215075

Purpose

Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRNA-Control

Plasmid

#98398

Purpose

Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

non-targeting randomized sequence

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pS34.EF1α<GFP-2A-Cas9-RC

Plasmid

#207404

Purpose

Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

Cas9-RC

Use

CRISPR and Synthetic Biology

Tags

GFP-2A

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.Tau2N4R

Plasmid

#226396

Purpose

Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EF.STAT3C.Ubc.GFP

Plasmid

#24983

Purpose

3rd generation lentiviral expression of constitutively active Stat3

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

A662C, N664C (constitutively active)

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

Plasmid

#217704

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGR6 (ADGRG6 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-840

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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Thy1.1-G5

Plasmid

#234839

Purpose

pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

Depositor

Gabriel Victora

Article

Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

Insert

Thy1.1 (Thy1 Mouse)

Use

Retroviral

Tags

EGFP-P2A-5xGly

Expression

Mammalian

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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