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pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Wnt7A STOP

Plasmid

#35877

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR4 TNRC6C

Plasmid

#20023

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Use

Entry vector

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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C413-E38: attB4-T7p>-attB1r

Plasmid

#162929

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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Wnt5A STOP

Plasmid

#35874

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEO100c

Plasmid

#50550

Purpose

Expresses sfGFP under the PompC1157 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 22, 2014

Availability

Academic Institutions and Nonprofits only
pTH-MYCN

Plasmid

#35416

Depositor

William A. Weiss

Article

Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN Human)

Expression

Mammalian

Promoter

Tryosine Hydroxlyase (TH)

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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C125-E01: stuffer

Plasmid

#162900

Purpose

Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265_OoA3GX2

Plasmid

#225147

Purpose

Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

OoA3GX2-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pME-luc2

Plasmid

#138355

Purpose

Gateway compatible middle entry clone contains Firefly Luciferase2 without minimal promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

luciferase

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Wnt4 STOP

Plasmid

#35873

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Entry vector

Mutation

Silent Mutation C (492) --> T

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-globin

Plasmid

#82582

Purpose

5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

Frog EF1a enhancer fused to rabbit b-globin intron

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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Wnt1 STOP

Plasmid

#35868

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBW400hrpL-gfp

Plasmid

#61437

Purpose

pBW400hrpL-gfp

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

hrpL-rbs30-gfp-ter

Expression

Bacterial

Promoter

HrpL

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Tags

HA

Mutation

um200, no stop codon; N1056S

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA1

Plasmid

#199727

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA1 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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