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pDONR221 hNLRP3-mNG-STOP

Plasmid

#218644

Purpose

Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Tags

mNeonGreen

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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BRAF - A12G in pENTR

Plasmid

#216606

Purpose

gateway cloning donor vector containing BRAF ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

BRAF (BRAF Human)

Use

Gateway entry plasmid

Mutation

A12G

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPTK003-2-pENO1

Plasmid

#84962

Purpose

Type 2, pENO1, Enolase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Enolase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (2350 g to c) and BsmBI site re…

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Wnt9B STOP

Plasmid

#35882

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Use

Entry vector

Mutation

SNP Met106-->Thr

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFTK043

Plasmid

#171315

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA3

Plasmid

#199729

Purpose

Golden Gate entry vector; 3rd gRNA under TaU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU3-sgRNA3 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA4

Plasmid

#199730

Purpose

Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C1-sgRNA4 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA5

Plasmid

#199731

Purpose

Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C3-sgRNA5 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA6

Plasmid

#199732

Purpose

Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.1-sgRNA6 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA7

Plasmid

#199733

Purpose

Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.2-sgRNA7 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA8

Plasmid

#199734

Purpose

Golden Gate entry vector; 8th gRNA under TaU6 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU6-sgRNA8 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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Wnt3 STOP

Plasmid

#35872

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt11 STOP

Plasmid

#35885

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

WNT11 (WNT11 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pKE014

Plasmid

#207908

Purpose

GG entry for zCas9i expression

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

SpCas9_introns_2xNLS-Pea3A(T) term

Use

CRISPR

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Joseph Davis, Robert Sauer

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Promoter

proD

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E26: attB2r-Halotag-attB3

Plasmid

#162915

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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