We narrowed to 5,573 results for: KRAS

1. pLVE-eGFP

   Plasmid

   #52581

   Purpose

   Lentiviral transfer vector driving expression of eGFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. C1-MPAct-mCherry

   Plasmid

   #155222

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. C1-MPAct-mRuby3

   Plasmid

   #155221

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
   

   Tags

   mRuby3

   Expression

   Mammalian

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1-MPAct-mCitrine

   Plasmid

   #155220

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
   

   Tags

   mCitrine

   Expression

   Mammalian

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2ADWpuro_2paRAF

   Plasmid

   #129653

   Purpose

   For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Expression

   Worm

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLG1-puro Non-targeting sgRNA 1

   Plasmid

   #109002

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK3b_031

   Plasmid

   #123809

   Purpose

   Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   KRAB:meCP2
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2ADW_2paRAF

    Plasmid

    #129654

    Purpose

    For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcold-6xHis-HRV3Csite-HsPACT

    Plasmid

    #41097

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    PACT (PRKRA Human)
    

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pFP140

    Plasmid

    #21672

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Mutation

    The second lacZ sequence has been modified, the E…

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pFP120

    Plasmid

    #21673

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Mutation

    The second lacZ sequence has been modified, a 918…

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pFP118

    Plasmid

    #21671

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Mutation

    The second lacZ sequence has been modified, the E…

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pLVUT-eC3GFP

    Plasmid

    #225485

    Purpose

    Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251, See Depositor Comments

    Promoter

    Human ubiquitin C

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK3b_022

    Plasmid

    #123803

    Purpose

    Encodes Kruppel Associated Box repression domain as a Type 3b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    KRAB
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK3a_014

    Plasmid

    #123766

    Purpose

    Encodes Kruppel Associated Box repression domain as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Krab
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFL-SV40-PACT 5'-UTR

    Plasmid

    #115351

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

    Depositor

    Ming-Chih Lai

    Article

    Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

    Insert

    PACT 5'-UTR (PRKRA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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