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pVoPo-mSc

Plasmid

#187842

Purpose

Constitutive mScarlet-I-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

mScarlet-I

Expression

Bacterial

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Article

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Tags

12X MS2 stem loops

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-tglB

Plasmid

#134676

Purpose

Expresses His6-TglBin E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglB

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pYL322d1/Vn-C

Plasmid

#183154

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pWS3799-gRNA-Csy4-template

Plasmid

#182827

Purpose

SpCas9 gRNA-Csy4 template

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

SpCas9 gRNA scaffold + Csy4 sequence

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLG025 (DUF4297 + HerA)

Plasmid

#157903

Purpose

Expresses the DUF4297 + HerA defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DUF4297 + HerA

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL6090

Plasmid

#193142

Purpose

Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Cbe ZTP Riboswitch sfGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

Deleted A26 to disable ligand-binding

Promoter

J23119

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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c-Rel cFlag pcDNA3

Plasmid

#20013

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

c-Rel (Rel Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pLG029 (mzaABCDE)

Plasmid

#157907

Purpose

Expresses the mzaABCDE defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

mzaABCDE (5-gene system containing a MutL homolog)

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLG030 (TerY-P)

Plasmid

#157908

Purpose

Expresses the TerY-P defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

TerY-P triad (vWA + kinase + phosphatase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

Plasmid

#217725

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE4

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-179

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKLBac-Bat-FluA-trimer

Plasmid

#229858

Purpose

Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

Inserts

PA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCW57.1-mDux-CA

Plasmid

#99284

Purpose

All-in-one lentivector with tet-inducible mouse Dux (codon sequences altered)

Depositor

Stephen Tapscott

Article

Whiddon et al Nat Genet. 2017 Jun;49(6):935-940. doi:

Insert

Dux-CA (mouse; codon altered) (Dux Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Promoter

SP6

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pmMaple3-CAM

Plasmid

#101148

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple3

Expression

Bacterial

Promoter

pBAD

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP

Plasmid

#64904

Purpose

"Fluorescent reporter containing no disulfide bonds/Selenocystamine folding"

Depositor

Stephen Mayfield

Article

Ferreira-Camargo et al AMB Express. 2015 Dec;5(1):126. doi: 10.

Insert

Green fluorescent protein

Use

Psba chlamydomonas reinhardtii vector

Promoter

psbA

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pKIKOrbsARCm

Plasmid

#46765

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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