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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 70,882 results for: LAS

Showing: 8311 - 8340 of 70882 results

  1. PP-PYRK1 (3KHD)

    Plasmid
    #25278
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFF1300w:A10-E511 (PFF1300w Plasmodium falciparum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A10-E511
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. p-dCas9-SET7-H297G-Hygro

    Plasmid
    #104415
    Purpose
    transient expression of dCas9-dSET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    dSET7
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H297G
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104415-plasmid-map-sequence-id-203185

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  3. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid
    #86456
    Purpose
    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86456-plasmid-map-sequence-id-171156

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  4. pAG471 Lyn11-iFAST

    Plasmid
    #130817
    Purpose
    Expresses Lyn11-iFAST in mammalian cells (inner plasma membrane labeling)
    Depositor
    Arnaud Gautier
    Insert
    Lyn11-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130817-plasmid-map-sequence-id-254955

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  5. PHIPA

    Plasmid
    #74665
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIPA (PHIP Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. PP-ChK

    Plasmid
    #25287
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0020:K80-D440 (PF14_0020 Plasmodium Falciparum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids K80-D440
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pLenti6.3_HES7

    Plasmid
    #124075
    Purpose
    HES7 with normal stop codon
    Depositor
    J. Chad Brenner
    Insert
    HES7 (HES7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124075-plasmid-map-sequence-id-241458

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  8. pBS ZfHoxb3a

    Plasmid
    #27522
    Depositor
    Victoria Prince
    Insert
    Hoxb3a (hoxb3a Zebrafish)
    Use
    Riboprobe synthesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. pBS ZfHoxb5a

    Plasmid
    #27524
    Depositor
    Victoria Prince
    Insert
    Hoxb5a (hoxb5a Zebrafish)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. 4MYJ

    Plasmid
    #60104
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0346:MAC04G-A11:C228843
    Use
    Baculovirus expression
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    See comments
    Promoter
    Polyhedrin
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. p404GALL

    Plasmid
    #17418
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GALL promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17418-plasmid-map-sequence-id-8593

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  12. pBS ZfHoxb6a

    Plasmid
    #27525
    Depositor
    Victoria Prince
    Insert
    Hoxb6a (hoxb6a Zebrafish)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. pBS ZfHoxa1a

    Plasmid
    #27513
    Depositor
    Victoria Prince
    Insert
    Hoxa1a (hoxa1a Zebrafish)
    Use
    Riboprobe production
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. PP-UBC13 (2R0J)

    Plasmid
    #25114
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFE1350c:C34884 (PFE1350c Plasmodium falciparum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 3-150
    Promoter
    Available since
    Feb. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pKlf4_Halo_Ty2_BactBsd

    Plasmid
    #235512
    Purpose
    C-term HALO targeting construct
    Depositor
    Brian Hendrich
    Insert
    Klf4 (Klf4 Mouse)
    Use
    Mouse Targeting
    Tags
    HALO, 2xTy
    Expression
    Mutation
    None
    Promoter
    beta actin
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235512-plasmid-map-sequence-id-474318

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  16. pML107-NET1

    Plasmid
    #232893
    Purpose
    Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    NET1 gRNA (NET1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232893-plasmid-map-sequence-id-470393

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  17. pML107-VRG4

    Plasmid
    #232899
    Purpose
    Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VRG4 gRNA (VRG4 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232899-plasmid-map-sequence-id-471178

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  18. pML107-SAM50

    Plasmid
    #232895
    Purpose
    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SAM50 gRNA (SAM50 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232895-plasmid-map-sequence-id-470389

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  19. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

    Plasmid
    #234382
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS
    Use
    Baculovirus expresssion
    Tags
    LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234382-plasmid-map-sequence-id-469458

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  20. pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233033
    Purpose
    To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233033-plasmid-map-sequence-id-464626

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  21. pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233034
    Purpose
    To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233034-plasmid-map-sequence-id-463768

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  22. pGL3_DDX25

    Plasmid
    #232359
    Purpose
    Enhancer near DDX25, chr11:125935511-125936012 cloned into pGL3
    Depositor
    Joanna Wysocka
    Insert
    DDX25 (DDX25 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232359-plasmid-map-sequence-id-463060

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  23. pGL3_MSR1_rightneg0topos1

    Plasmid
    #232389
    Purpose
    MSR1 enhancer, right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, right buffering Coordinator to sen…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232389-plasmid-map-sequence-id-462852

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  24. pML104-HygMx4-URA3-3

    Plasmid
    #232886
    Purpose
    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232886-plasmid-map-sequence-id-462214

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  25. pML104-HygMx4-URA3-2

    Plasmid
    #232885
    Purpose
    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232885-plasmid-map-sequence-id-462211

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  26. Lmir_cytGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228385
    Purpose
    Expresses N-term transit peptide from Lophophytum mirabile cytoplasmic Glutamate aminoacyl-tRNA synthetase (TRINITY_DN2194_c0_g1_i3); determination of L. mirabile cytoplasmic GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. mirabile cytoplasmic GluRS (TRINITY_DN2194_c0_g1_i3)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228385-plasmid-map-sequence-id-455876

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  27. Lpyr_cytGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228388
    Purpose
    Expresses N-term transit peptide from Lophophytum pyramidale cytoplasmic Glutamate aminoacyl-tRNA synthetase (TRINITY_DN180208_c0_g1_i5); to determine L. pyramidale cytoplasmic GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from L. pyramidale cytoplasmic GluRS (TRINITY_DN180208_c0_g1_i5)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228388-plasmid-map-sequence-id-455878

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  28. Balfun_cytGluRS_TP_pK7fWG2_IVD_FP611

    Plasmid
    #228382
    Purpose
    Expresses N-term transit peptide from Balanophora fungosa cytoplasmic Glutamate aminoacyl-tRNA synthetase (GWHDONK00049274); determination of B. fungosa cytoplasmic GluRS target localization
    Depositor
    Daniel Sloan
    Insert
    N-terminal transit peptide from B. fungosa cytoplasmic GluRS (GWHDONK00049274)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228382-plasmid-map-sequence-id-455874

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  29. MigR1 GFP Mouse PHGDH

    Plasmid
    #220591
    Purpose
    Retroviral mammalian expression vector with GFP for overexpression of mouse PHGDH
    Depositor
    Russell Jones
    Insert
    Ms PHGDH (Phgdh Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    220591-plasmid-map-sequence-id-436554

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  30. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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Showing: 8311 - 8340 of 70882 results