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CALR-i2-HT2

Plasmid

#236334

Purpose

Inducibly expresses CALR tagged with HaloTag2 at the relative location of intron 2 in the CALR gene.

Depositor

Ophir Shalem

Article

Sansbury et al bioRxiv. 2023 Jul 14:2023.07.13.548611.

Insert

CALR (CALR Human)

Use

Lentiviral

Tags

HaloTag2 internal insertion at intron 2

Expression

Mammalian

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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EPHA2-i16-HT2

Plasmid

#236335

Purpose

Inducibly expresses EPHA2 tagged with HaloTag2 at the relative location of intron 16 in the EPHA2 gene.

Depositor

Ophir Shalem

Article

Sansbury et al bioRxiv. 2023 Jul 14:2023.07.13.548611.

Insert

EPHA2 (EPHA2 Human)

Use

Lentiviral

Tags

HaloTag2 internal insertion at intron 16

Expression

Mammalian

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pcdna3_HA-KLC1(TPR6)

Plasmid

#252745

Purpose

Expression of the TPR repeats of KLC1 which can act as a dominant negative inhibitor of kinesin-1 function.

Depositor

Tom Rapoport, Kristen Verhey

Article

Verhey et al J Cell Biol. 2001 Mar 5;152(5):959-70. d

Insert

KLC1 (Klc1 Rat)

Tags

HA

Expression

Mammalian

Mutation

TPR region only

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pFlagTurboNTD(C1)-c20orf27

Plasmid

#253075

Purpose

Expression of FlagTurboNTD-c20orf27

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

c20orf27 (C20orf27 Human)

Tags

FlagTurboNTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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ClhN-P1K-3VSV-Atg8(T56A T87A)-MET15

Plasmid

#207048

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

T56A T87A

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(P85A P86A)-MET15

Plasmid

#207049

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

P85A P86A

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pQβEP1

Plasmid

#210859

Purpose

Express phage Qβ with SARS-CoV Spike protein epitope 1 displayed on the surface that can bind anti-S antibodies under T7 promotor

Depositor

Alain Bopda Waffo

Article

Sanders et al J Biol Chem. 2023 Dec;299(12):105460. do

Insert

SARS-CoV Spike AA441-460 (S )

Tags

No

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pQβEP3

Plasmid

#210861

Purpose

Express phage Qβ with SARS-CoV Spike protein epitope 3 displayed on the surface that can bind anti-S antibodies under T7 promotor

Depositor

Alain Bopda Waffo

Article

Sanders et al J Biol Chem. 2023 Dec;299(12):105460. do

Insert

SARS-CoV Spike AA781-800 (S )

Tags

No

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E53TAG)-mCherry

Plasmid

#241293

Purpose

Mammalian expression of syntaxin-1(E53TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E206TAG)-mCherry

Plasmid

#241295

Purpose

Mammalian expression of syntaxin-1(E206TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E206TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K84TAG)-mCherry

Plasmid

#241297

Purpose

Mammalian expression of syntaxin-1(K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E49TAG)-mCherry

Plasmid

#241291

Purpose

Mammalian expression of syntaxin-1(E49TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPIC9K_A2AR_316_V229C_R293A

Plasmid

#246918

Purpose

Express A2AR mutants in Pichia Pastoris

Depositor

Libin Ye

Article

Wang et al Nat Commun. 2023 Mar 10;14(1):1325. doi:

Insert

Adenosine A2A receptor (ADORA2A Human)

Tags

10xHis and alpha-factor secretion signal, FLAG

Expression

Yeast

Mutation

truncated to aa 1-316, V229C, R293A

Promoter

AOX1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pPIC9K_A2AR_316_V229C_R291AR293A

Plasmid

#246919

Purpose

Express A2AR mutants in Pichia Pastoris

Depositor

Libin Ye

Article

Wang et al Nat Commun. 2023 Mar 10;14(1):1325. doi:

Insert

Adenosine A2A receptor (ADORA2A Human)

Tags

10xHis and alpha-factor secretion signal, FLAG

Expression

Yeast

Mutation

truncated to aa 1-316, V229C, R291A, R293A

Promoter

AOX1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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ER-GFP pLVX CMVpuro

Plasmid

#246912

Purpose

Lentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2-3_005_Promoter+5'UTR_Placeholder

Plasmid

#235870

Purpose

Encodes a sfGFP cassette in the promoter+5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter + 5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_D11 (pAVA3773)

Plasmid

#239310

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and a non-targeting sgRNA as control(-)

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven non-targeting sgRNA Control(-)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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