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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_GST-Nb32

Plasmid

#228895

Purpose

Express GST-tagged anti-Zika Nb32 in E.coli

Depositor

Magnus Rueping

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

GST-tagged Nb32

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_GST-NbD6

Plasmid

#228896

Purpose

Express GST-tagged anti-Zika NbD6 in E.coli

Depositor

Magnus Rueping

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

GST-tagged NbD6

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJEx411c_pelB-NbD6-Strep2

Plasmid

#228897

Purpose

Periplasmic Expresssion of Strep-tagged anti-Zika NbD6

Depositor

Stefan Arold

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

sb0379

Tags

Strep2

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJEx411c_pelB-N32-Strep2

Plasmid

#228898

Purpose

Periplasmic Expresssion of Strep-tagged anti-Zika NbD32

Depositor

Stefan Arold

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

sb0380

Tags

Strep2

Expression

Bacterial

Promoter

T7

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)

Plasmid

#231603

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA

Plasmid

#231604

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized-8xAAA

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot

Plasmid

#231593

Purpose

Express circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-2xALFATag-10xSunTag-Pseudoknot sequence

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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GB4653

Plasmid

#215267

Purpose

TU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

pNos:FLP:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GB4829

Plasmid

#215275

Purpose

TU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:NPGA:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-C5

Plasmid

#209420

Purpose

Express anti-SARS-CoV-2-Nanobody C5 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-C5

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-F2

Plasmid

#209421

Purpose

Express anti-SARS-CoV-2-Nanobody F2 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-F2

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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OSCA1.2-K435L-peGFP-N1

Plasmid

#228586

Purpose

Expresses OSCA1.2-K435L-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM63A-S475K-peGFP-N1

Plasmid

#228592

Purpose

Expresses mouse TMEM63A-S475K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Expression

Mammalian

Mutation

S475K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM63A-A476K-peGFP-N1

Plasmid

#228593

Purpose

Expresses mouse TMEM63A-A476K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Expression

Mammalian

Mutation

A476K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-A439K-peGFP-N1

Plasmid

#228589

Purpose

Expresses OSCA1.2-A439K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

A439K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDIV302

Plasmid

#204973

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
nat1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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GB3774

Plasmid

#215247

Purpose

CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CPH

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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