We narrowed to 10,337 results for: plasmids 101

1. pAAV-FLEX-SaCas9-U6-sgSlc17a8

   Plasmid

   #124848

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-3xGFP11-mem-3xControlgRNA

   Plasmid

   #224583

   Purpose

   Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   3xGFP11
   

   Use

   CRISPR

   Tags

   Membrane Localization Signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_SNIPR_hsNotch3 LNR

   Plasmid

   #188338

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_PGK_SNIPR_FAP site with scFv

   Plasmid

   #188351

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPC019.AAV

   Plasmid

   #171147

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIZ-turq-3xFLAG-TREX1 D18N

   Plasmid

   #164233

   Purpose

   retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and mturquoise

   Expression

   Mammalian

   Mutation

   D18N ->nuclease deficient

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1-GT_CAG_3xTurboGFP-NLS

   Plasmid

   #229777

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   3x TurboGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS183

   Plasmid

   #134142

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hGFAP-NEUROD1-T2A-mCherry

   Plasmid

   #178582

   Purpose

   Expression of NEUROD1 and mCherry under the human GFAP promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-LAP2-mCherry

    Plasmid

    #159524

    Purpose

    Expresses mCherry from LAP2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LAP2

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2pA2-mnx1:MLS-KillerRed

    Plasmid

    #115515

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    -3mnx1

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_PGK_SNIPR_FnIII-Notch

    Plasmid

    #188342

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_FnIII-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-mas-KB-1753-Nluc

    Plasmid

    #158603

    Purpose

    Plasmid expressing KB-1753-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-beta

    Plasmid

    #169240

    Purpose

    Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETcLIC-GFP

    Plasmid

    #183514

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19_dTAG-KANSL3-targeting

    Plasmid

    #170755

    Purpose

    Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    BSD-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    BSD-P2A-2xHA-dTAG

    Expression

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBK1290-AAV-dSaCas9

    Plasmid

    #223139

    Purpose

    AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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