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Showing: 11121 - 11160 of 170823 results

  1. pREP42xV5_ccdB

    Plasmid
    #79018
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79018-plasmid-map-sequence-id-147775

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  2. Plekhm1 - C terminal pFastHFTb

    Plasmid
    #73829
    Purpose
    Express human Plekhm1 C-part in insect cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Tags
    6xHis and Flag
    Expression
    Insect
    Mutation
    cloned C-terminal part of human Plekhm1 (aa 493- …
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. Plekhm1-N terminal pFastHFTb

    Plasmid
    #73828
    Purpose
    Express human Plekhm1 N-part in insect cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Tags
    6xHis and Flag
    Expression
    Insect
    Mutation
    cloned N- terminal part of human Plekhm1 (aa 1- 4…
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pJK561

    Plasmid
    #72283
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Expression
    Bacterial
    Mutation
    changes cysteine-35 to serine
    Promoter
    T7
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72283-plasmid-map-sequence-id-131874

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  5. pJK523

    Plasmid
    #72442
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72442-plasmid-map-sequence-id-133863

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  6. R777-E280 Hs.RHOB-nostop

    Plasmid
    #70564
    Purpose
    Gateway ORF clone of human RHOB [NM_004040.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RHOB (RHOB Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70564-plasmid-map-sequence-id-126013

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  7. R777-E210 Hs.RAC2-nostop

    Plasmid
    #70494
    Purpose
    Gateway ORF clone of human RAC2 [NM_002872.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RAC2 (RAC2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70494-plasmid-map-sequence-id-125943

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  8. R777-E192 Hs.PRKAA1-nostop

    Plasmid
    #70476
    Purpose
    Gateway ORF clone of human PRKAA1 [NM_006251.5] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAA1 (PRKAA1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70476-plasmid-map-sequence-id-125925

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  9. R777-E176 Hs.PIK3R3-nostop

    Plasmid
    #70460
    Purpose
    Gateway ORF clone of human PIK3R3 [NM_003629.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R3 (PIK3R3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70460-plasmid-map-sequence-id-125909

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  10. pJK138

    Plasmid
    #73314
    Purpose
    Pas2p peroxisomal membrane targeting sequence
    Depositor
    T. J. Kappock
    Insert
    Pas2p (peroxisomal membrane targeting region)
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    encodes residues 1-42
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73314-plasmid-map-sequence-id-138890

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  11. pJK126

    Plasmid
    #73337
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)
    Depositor
    T. J. Kappock
    Insert
    electron transfer flavoprotein
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 328-685
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73337-plasmid-map-sequence-id-135048

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  12. pJK323

    Plasmid
    #73495
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73495-plasmid-map-sequence-id-135755

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  13. pJK366

    Plasmid
    #72405
    Purpose
    Produces Acetobacter aceti 1023 Est02
    Depositor
    T. J. Kappock
    Insert
    peptidase S9
    Expression
    Bacterial
    Mutation
    additional N-terminal MVKNGS sequence, alternate …
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72405-plasmid-map-sequence-id-135520

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  14. pJK377

    Plasmid
    #72411
    Purpose
    Produces Acetobacter aceti 1023 Est05
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72411-plasmid-map-sequence-id-135530

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  15. pJK322

    Plasmid
    #73494
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Promoter
    n/a
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73494-plasmid-map-sequence-id-135746

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  16. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

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  17. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

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  18. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

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  19. pJK684

    Plasmid
    #71638
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71638-plasmid-map-sequence-id-131658

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  20. pJK685

    Plasmid
    #71639
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71639-plasmid-map-sequence-id-131659

    Enlarge

  21. pJK687

    Plasmid
    #71640
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71640-plasmid-map-sequence-id-131661

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  22. pJK688

    Plasmid
    #71641
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71641-plasmid-map-sequence-id-131662

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  23. pJK695

    Plasmid
    #71644
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71644-plasmid-map-sequence-id-131665

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  24. pJK696

    Plasmid
    #71645
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71645-plasmid-map-sequence-id-131666

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  25. pJK607

    Plasmid
    #71626
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys mutant (TdPurE2-S38C)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to cysteine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71626-plasmid-map-sequence-id-131645

    Enlarge

  26. pJK617

    Plasmid
    #71627
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala mutant (TdPurE2-S38A)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to alanine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71627-plasmid-map-sequence-id-131646

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  27. pJK643

    Plasmid
    #71628
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to threonine
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71628-plasmid-map-sequence-id-131647

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  28. pJK644

    Plasmid
    #71629
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71629-plasmid-map-sequence-id-131648

    Enlarge

  29. pJK125

    Plasmid
    #73336
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)
    Depositor
    T. J. Kappock
    Insert
    electron transfer flavoprotein
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 1-327
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73336-plasmid-map-sequence-id-138202

    Enlarge

  30. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  31. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

    Enlarge

  32. pJK637

    Plasmid
    #72437
    Purpose
    Acetobacter aceti 1023 mutS, partial gene
    Depositor
    T. J. Kappock
    Insert
    DNA mismatch repair protein MutS (partial gene)
    Expression
    Bacterial
    Mutation
    deletes nt 1-767 and nt 2086-2655 of mutS gene
    Promoter
    n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72437-plasmid-map-sequence-id-132658

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  33. pJK530

    Plasmid
    #72511
    Purpose
    Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster with C-terminal His6 tag (AaOrfYH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein in purSQL operon
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72511-plasmid-map-sequence-id-132537

    Enlarge

  34. pJK533

    Plasmid
    #72514
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72514-plasmid-map-sequence-id-135820

    Enlarge

  35. pJK575

    Plasmid
    #72526
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72526-plasmid-map-sequence-id-132611

    Enlarge

  36. pJK620

    Plasmid
    #72537
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6-tag (H6AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72537-plasmid-map-sequence-id-135983

    Enlarge

  37. pJK622

    Plasmid
    #72538
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit with N-terminal His6-tag (H6AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72538-plasmid-map-sequence-id-132629

    Enlarge

  38. pJK590

    Plasmid
    #71698
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag (AaTrxB1H6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71698-plasmid-map-sequence-id-131731

    Enlarge

  39. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

    Enlarge

  40. pRP-iSL(MCS1+2)

    Plasmid
    #69245
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Promoter
    RRNA promoter
    Available Since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 11121 - 11160 of 170823 results