We narrowed to 17,015 results for: emb

1. EGFP-PIP5K1A

   Plasmid

   #202720

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   EGFP:PIP5K1A (PIP5K1A Human)
   

   Expression

   Mammalian

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUV15-pHGFP

   Plasmid

   #70045

   Purpose

   encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

   Depositor

   Sabine Ehrt

   Article

   Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

   Insert

   phGFP
   

   Use

   Mycobacterium tuberculosis expression vector

   Promoter

   mycobacterial promoter Psmyc

   Available Since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUS_B2

   Plasmid

   #31019

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Blast-miRFP670nano3

   Plasmid

   #207680

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMetYC-Dest

   Plasmid

   #105081

   Purpose

   Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Tags

   Cub-PLV

   Expression

   Yeast

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-CFP

   Plasmid

   #25998

   Depositor

   Elaine Fuchs

   Article

   Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

   Insert

   H2B-CFP (H2BC21 A. victoria, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOPO mCherry

   Plasmid

   #68490

   Purpose

   TOPO construct expressing mCherry for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mCherry
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEnt L1L3 EF1a-tTA-2

   Plasmid

   #26803

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Elongation Factor 1 alpha promoter and tTA transgene
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6.3>gRNA.f+e

   Plasmid

   #99139

   Purpose

   Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Promoter

   chicken U6.3

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LgBiT-ST

    Plasmid

    #207633

    Purpose

    A plasmid encoding LgBiT fused to SpyTag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    LgBiT-SpyTag
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-ultraID-Blast

    Plasmid

    #207715

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCMU-GAr

    Plasmid

    #61172

    Purpose

    Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    GmMAN49-mCherry
    

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Reb_206 aka PAS629

    Plasmid

    #71234

    Purpose

    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    mNeonGreen to second copy RebB, soluble mCherry a…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST/LIfeAct-mCherry-N1

    Plasmid

    #40908

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    LifeAct
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSV2 neuNT

    Plasmid

    #10918

    Depositor

    Bob Weinberg

    Article

    Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

    Insert

    neuNT (Erbb2 Rat)
    

    Expression

    Mammalian

    Available Since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2699-CMV-Nme2Cas9-E932R

    Plasmid

    #228720

    Purpose

    Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-E932R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid

    #228717

    Purpose

    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-D56R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid

    #228718

    Purpose

    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-E932R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS2698-CMV-Nme2Cas9-D56R

    Plasmid

    #228719

    Purpose

    Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-D56R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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