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We narrowed to 6,470 results for: val

Showing: 271 - 280 of 6470 results
  1. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

    Plasmid
    #169298
    Purpose
    Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Insert
    Arid3a-3'UTR-Mut (Arid3a Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

    Plasmid
    #169296
    Purpose
    Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Insert
    ARID3A-3'UTR-Mut (ARID3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pHybr_r2a>mG2b-srt-his

    Plasmid
    #124804
    Purpose
    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.
    Insert
    Murine IgG2b
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid
    #207357
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Insert
    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 271 - 280 of 6470 results