We narrowed to 2,230 results for: Kap

1. pRDB_092

   Plasmid

   #231110

   Purpose

   C>T base editor

   Depositor

   John Doench, David Root

   Insert

   TadA-CDd-nCas9-SpG-UGI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA516

   Plasmid

   #231118

   Purpose

   Fragmid fragment: (2A Selection) CBE activity-based selection intron

   Depositor

   John Doench, David Root

   Insert

   PuroN'-intron-PuroC'
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA509

   Plasmid

   #231116

   Purpose

   Fragmid fragment: (2A Selection) ABE activity-based selection intron

   Depositor

   John Doench, David Root

   Insert

   PuroN'-intron-PuroC'
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK652

   Plasmid

   #72470

   Purpose

   Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)

   Depositor

   T. J. Kappock

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated histidine-233 to glycine

   Promoter

   T7

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK288

   Plasmid

   #71552

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with Tyr260>Ala mutant (AaAlr-Y260A)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tyrosine-260 to alanine

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK301

   Plasmid

   #71555

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with C-terminal His tag (AaAlrH6)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK520

   Plasmid

   #72479

   Purpose

   Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   

   Use

   Gene synthesis

   Tags

   His6

   Expression

   Mutation

   synthetic gene

   Promoter

   n/a and none

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK559

   Plasmid

   #71603

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, tyrosine-…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK562

   Plasmid

   #71604

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, tyrosine-…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK604

    Plasmid

    #71610

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK664

    Plasmid

    #71613

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine, tyrosine-154 …

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK666

    Plasmid

    #71615

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine, tryptophan-97…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK563

    Plasmid

    #71689

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tyrosine-…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK508

    Plasmid

    #71598

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCVD529

    Plasmid

    #66159

    Purpose

    A 2.7 kb HindIII fragment carrying the 2nd tdh gene copy was cloned from the chromosome of Vibrio parahemolyticus strain WP1 into the HindIII site of pBR322

    Depositor

    James Kaper

    Article

    Nishibuchi et al Mol Microbiol. 1990 Jan;4(1):87-99.

    Insert

    tdh2
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pTK234

    Plasmid

    #13535

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTTY
    

    Use

    Tags

    See map

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTK222

    Plasmid

    #13532

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTYT
    

    Use

    Tags

    See map

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMX IkB

    Plasmid

    #12331

    Depositor

    Inder Verma

    Article

    Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

    Insert

    IkB alpha (Nfkbia Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pJK295

    Plasmid

    #71561

    Purpose

    Production of Acetobacter aceti 1023 thioredoxin (AaTrx)

    Depositor

    T. J. Kappock

    Article

    Starks et al Protein Sci. 2007 Jan;16(1):92-8.

    Insert

    thioredoxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLIPmal-40uDelta1YFP

    Plasmid

    #18853

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Insert

    optimized maltose FRET sensor
    

    Use

    Tags

    CFP and YFP

    Expression

    Bacterial

    Mutation

    N227A;

    Promoter

    Available since

    Oct. 23, 2008

    Availability

    Academic Institutions and Nonprofits only