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pDest17_Gcn4 Aro3+

Plasmid

#231884

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Aro2+

Plasmid

#231883

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WTSLFD

Plasmid

#231881

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WTSLFD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-HA-TEADiv2

Plasmid

#228875

Purpose

Gateway entry vector for optimized 2xHA-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Gateway entry vector

Tags

2xHA

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-EGFP-TEADiv2

Plasmid

#228874

Purpose

Gateway entry vector for optimized GFP-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Gateway entry vector

Tags

GFP

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-peGFP-N1

Plasmid

#228586

Purpose

Expresses OSCA1.2-K435L-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM63A-S475K-peGFP-N1

Plasmid

#228592

Purpose

Expresses mouse TMEM63A-S475K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Expression

Mammalian

Mutation

S475K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM63A-A476K-peGFP-N1

Plasmid

#228593

Purpose

Expresses mouse TMEM63A-A476K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Expression

Mammalian

Mutation

A476K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-A439K-peGFP-N1

Plasmid

#228589

Purpose

Expresses OSCA1.2-A439K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

A439K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-PARP7

Plasmid

#184474

Purpose

For gateway cloning of PARP7

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP7 (TIPARP Human)

Use

Gateway cloning

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-PARP15

Plasmid

#184471

Purpose

For gateway cloning of PARP15

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP15 (PARP15 Human)

Use

Gateway cloning

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225704

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mTurquoise

Expression

Mammalian

Promoter

pEF1A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-VP64-2A-mCherryBSD

Plasmid

#225695

Purpose

Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-VP64

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mCherry

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-KRAB-2A-mTurquoise

Plasmid

#225690

Purpose

Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-ZNF10 KRAB

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideSLC35C1-neo

Plasmid

#220868

Purpose

encodes CRISPR sgRNA targeting human SLC3C1

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

SLC35C1 (SLC35C1 Human)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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