We narrowed to 18,681 results for: tec

1. pAF0689 Ef1a-Pa01_attB-BFP acceptor

   Plasmid

   #193472

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3XFlagNICD4

   Plasmid

   #20186

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 4 Intracellular Domain (Notch4 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 4 Intracellular Domain (Val…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pGWB455

   Plasmid

   #74837

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAS1yl

   Plasmid

   #73226

   Purpose

   Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

   Depositor

   Sheng Yang

   Article

   Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR; In y. lipolytica

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   unknown

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCALNL-NaChBac

   Plasmid

   #125580

   Purpose

   Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   NaChBac
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-ZoAl-RTD-3xF-ORF

   Plasmid

   #238486

   Purpose

   For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   DD644/645->AA

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-ZoAl-END-3xF-ORF

   Plasmid

   #238485

   Purpose

   For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   DD1041/1054->AA

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-TaGu-RTD-3xF-ORF

   Plasmid

   #238490

   Purpose

   For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   DD660/661->AA

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-TaGu-END-3xF-ORF

   Plasmid

   #238489

   Purpose

   For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   DD1057/1070->AA

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEL035

    Plasmid

    #137903

    Purpose

    STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115209

    Purpose

    Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PbuCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.4_17G6-1 heavy chain

    Plasmid

    #198249

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGWB555

    Plasmid

    #74885

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115216

    Purpose

    Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin2Cas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB405

    Plasmid

    #74799

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB441

    Plasmid

    #74827

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid

    #181783

    Purpose

    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115207

    Purpose

    Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    BzoCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge