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P2A-CreER-pA-ORF2-FRT-acry mCherry

Plasmid

#241168

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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P2A-CreER-pA-ORF3-FRT-acry mCherry

Plasmid

#241169

Purpose

Middle entry vector for mini-Golden subcloning platform; creER

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

creER

Use

Mini-golden

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVSΔREP-RT-pDGB3alpha1

Plasmid

#240727

Purpose

BCTV vector containing the GUS:NPTII repair template for HDR experiments. Lacks the BCTV virion sense ORFs and Rep has been mutated.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF3

Plasmid

#241182

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

Plasmid

#241191

Purpose

Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

3x-FLAG; mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

Plasmid

#241190

Purpose

Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

V5-APEX2n; mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF3

Plasmid

#241194

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF2

Plasmid

#241193

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF1

Plasmid

#241192

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1

Plasmid

#241180

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF2

Plasmid

#241181

Purpose

Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

NTRv2; mNeonGreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Plasmid

#233424

Purpose

IVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-nmba

Plasmid

#184821

Purpose

Used to synthesize RNA probe for in situ hybridization for nmba

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

nmba (nmba Zebrafish)

Use

Template for synthesis of nmba probe

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-min5UTR-hRluc-A60

Plasmid

#182638

Purpose

Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase,

Use

Luciferase

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199450

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199453

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

Plasmid

#199451

Purpose

CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

Plasmid

#199452

Purpose

CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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