We narrowed to 16,540 results for: puromycin

1. lentiGuide-SYNJ2BP-2

   Plasmid

   #92160

   Purpose

   CRISPR guide RNA targeting human SYNJ2BP

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   SYNJ2BP sgRNA-2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLI_C-tag TNF

   Plasmid

   #171178

   Purpose

   inducible expression of C-tag TNF

   Depositor

   Veit Hornung

   Article

   Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

   Insert

   C-tag TNF
   

   Use

   Lentiviral

   Tags

   C-tag (internal)

   Expression

   Mammalian

   Mutation

   ADAM cleavage site of TNF (AA73-76) was exchanged…

   Promoter

   tight TRE promoter

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-PG13-mCherry-EF1a-PuroR-EGFP

   Plasmid

   #195292

   Purpose

   piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

   Depositor

   Hideyuki Oguro

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   mCherry
   PuroR-P2A-EGFP
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

   Plasmid

   #68375

   Purpose

   Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and TALEN; Zfn

   Tags

   3xFLAG_Twin_Strep

   Expression

   Mammalian

   Promoter

   human PGK1

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-GAL9

   Plasmid

   #166689

   Purpose

   For creating a stable mCherry-GAL9 cell line

   Depositor

   Alan Sabirsh

   Article

   Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

   Insert

   LGALS9 (LGALS9 Human)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-U6-sgRNA-EGFP

   Plasmid

   #107721

   Purpose

   For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176273

   Purpose

   Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (sgTet) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-TetOne-Puro-LUC

   Plasmid

   #218630

   Purpose

   This plasmid contains luciferase under doxycycline inducible control.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   LUC (LOC116160065 )
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi 41BBL-CD86 mIL-15

    Plasmid

    #183245

    Purpose

    Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Human, Synthetic)
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-puro-GFP-progerin

    Plasmid

    #17663

    Depositor

    Tom Misteli

    Article

    Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

    Insert

    GFP-D50 lamin A (LMNA Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta 50

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM705 Gs

    Plasmid

    #109350

    Purpose

    Wildtype Gαs

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    GαS (GNAS Human)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHage2-EF1a-TERT-IRES-Puro

    Plasmid

    #182590

    Purpose

    expresses TERT in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LT3GEPIR-WT1shRNA

    Plasmid

    #220560

    Purpose

    To inducibly knockdown WT1 or EWSR1::WT1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    WT1 shRNA (WT1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. V5-LOV-Turbo-NLS

    Plasmid

    #199663

    Purpose

    expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NLS and V5

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPCX-Cx43-IRES-GFP

    Plasmid

    #65433

    Purpose

    Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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