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pEF mito-cGFP (matrix)

Plasmid

#188904

Purpose

Expresses mitochondrial GFP

Depositor

Edwin Chapman

Publication

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mitochondrial GFP

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Expression

Mammalian

Mutation

none

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-E2F3

Plasmid

#202522

Purpose

Expressing human E2F3 protein with FLAG-tag

Depositor

Ming Chen

Publication

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

E2F3 (E2F3 Human)

Tags

FLAG-tag

Expression

Mammalian

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Publication

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSec61β-mScarlet3-H (aka mYongHong)

Plasmid

#239414

Purpose

Expresses Sec61b-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Publication

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec61β (SEC61B Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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tetO-FOSL1

Plasmid

#170687

Purpose

doxycycline-inducible overexpression of FOSL1

Depositor

Paul Burridge

Publication

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

FOSL1 (FOSL1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TERT

Plasmid

#156078

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TERT (TERT Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Tubulin-LSSmGFP

Plasmid

#186535

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LSSmGFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ40-GFP-iBox-PAK4cat

Plasmid

#190179

Purpose

expresses GFP-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Publication

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP-iBox-PAK4cat

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ECD

Plasmid

#121479

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Publication

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Mutation

ECD+TM

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ICD

Plasmid

#121480

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Publication

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Mutation

TM+ICD

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Publication

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TRIM25_G1

Plasmid

#127119

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA TRIM25 (TRIM25 Human)

Use

CRISPR

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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JL002: pMVP/eGFP-DEST

Plasmid

#121852

Purpose

pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG

Plasmid

#127301

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Publication

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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